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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Susceptibility of Candida albicans, Staphylococcus aureus, and Streptococcus mutans biofilms to photodynamic inactivation: an in vitro study

Texto completo
Autor(es):
Pereira, Cristiane Aparecida [1] ; Romeiro, Rogerio Lima [1] ; Borges Pereira Costa, Anna Carolina [1] ; Silva Machado, Ana Karina [1] ; Junqueira, Juliana Campos [1] ; Cardoso Jorge, Antonio Olavo [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] UNESP Univ Estadual Paulista, Sch Dent Sao Jose Campos, Dept Biosci & Oral Diag, BR-12245000 Sao Dimas, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Lasers in Medical Science; v. 26, n. 3, p. 341-348, MAY 2011.
Citações Web of Science: 108
Resumo

The purpose of this study was to evaluate specific effects of photodynamic inactivation (PDI) using methylene blue as photosensitizer and low-power laser irradiation on the viability of single-, dual-, and three-species biofilms formed by C. albicans, S. aureus, and S. mutans. Biofilms were grown in acrylic discs immersed in sterile brain heart infusion broth (BHI) containing 5% sucrose, inoculated with microbial suspension (10(6) cells/ml) and incubated for 5 days. On the fifth day, the effects of the methylene blue (MB) photosensitizer at a concentration of 0.1 mg/ml for 5 min and InGaAlP laser (660 nm) for 98 s, alone and conjugated were evaluated. Next, the discs were placed in tubes with sterile physiological solution {[}0.9% sodium chloride (NaCl)] and sonicated for to disperse the biofilms. Ten-fold serial dilutions were carried and aliquots seeded in selective agar, which were then incubated for 48 h. Then the numbers CFU/ml (log(10)) were counted and analyzed statistically (ANOVA, Tukey test, p < 0.05). Scanning electron microscopy (SEM) on discs treated with PDI and control biofilms groups was performed. Significant decreases in the viability of all microorganisms were observed for biofilms exposed to PDI mediated by MB dye. Reductions (log(10)) of single-species biofilms were greater (2.32-3.29) than the association of biofilms (1.00-2.44). Scanning electron microscopy micrographs suggested that lethal photosensitization occurred predominantly in the outermost layers of the biofilms. The results showed that PDI mediated by MB dye, might be a useful approach for the control of oral biofilms. (AU)

Processo FAPESP: 09/12005-1 - Avaliação da terapia fotodinâmica em Candida albicans in vitro e in vivo
Beneficiário:Anna Carolina Borges Pereira da Costa
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 09/52048-1 - Efeitos da terapia fotodinâmica e de óleos essenciais de Cymbopogon citratus (DC.) Stapf, Casearia sylvestris SW e Curcuma zedoaria Roscoe, in vitro, em biofilmes formados com Candida albicans, Staphylococcus aureus e Streptococcus mutans
Beneficiário:Antonio Olavo Cardoso Jorge
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 10/00879-4 - Microrganismos oportunistas em lesões de estomatite protética e influência da terapia fotodinâmica nos fatores de virulência de Candida spp
Beneficiário:Cristiane Aparecida Pereira
Linha de fomento: Bolsas no Brasil - Doutorado