| Texto completo | |
| Autor(es): |
Zuliani, Carolina Coli
;
da Cunha, Jessica Bruna
;
Correa, Rodolpho Fagundes
;
Pinto Junior, Eronildo Alves
;
d'Avila, Marcos Akira
;
Moraes, Angela Maria
;
Coimbra, Ibsen Bellini
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY; v. 53, n. 1, p. 15-pg., 2025-12-31. |
| Resumo | |
Cartilage injuries caused by trauma or degenerative conditions such as osteoarthritis have limited intrinsic healing capacity and often require invasive interventions. This study proposes a minimally invasive therapeutic strategy using spheroids of human amniotic fluid-derived mesenchymal stromal cells (AF-MSCs) embedded in a thermosensitive hydrogel composed of 10% (w/v) methylcellulose and 1% (w/v) carboxymethyl chitosan (M10C1). AF-MSCs spheroids, generated in 3D culture under TGF-beta 3 stimulation, exhibited high viability and robust extracellular matrix production, including type II collagen and aggrecan. Rheological characterisation confirmed that M10C1 displays shear-thinning behaviour and gelation near physiological temperatures (similar to 30 degrees C), making it suitable for injection and 3D bioprinting. Importantly, confocal microscopy and fusion assays demonstrated that the hydrogel preserved spheroid bioassembly and viability. Co-culture with human cartilage explants further showed that M10C1 promoted spheroid adhesion without hindering integration into native tissue. These findings highlight the potential of this AF-MSCs spheroid-hydrogel system as an injectable, biocompatible platform for cartilage repair, with promising applications in regenerative medicine and bioengineered tissue therapies. (AU) | |
| Processo FAPESP: | 24/05735-3 - Hidrogéis Multifuncionais para Injeção Intra-articular e Bioimpressão 3D de Construtos Aplicados na Engenharia Tecidual da Cartilagem Hialina |
| Beneficiário: | Rodolpho Fagundes Correa |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 22/09275-1 - Cultivo e diferenciação condrogênica de células tronco em modelo 3D |
| Beneficiário: | Jéssica Bruna da Cunha |
| Modalidade de apoio: | Bolsas no Brasil - Programa Capacitação - Treinamento Técnico |
| Processo FAPESP: | 21/14738-8 - Diferenciação condrogênica de células tronco de líquido amniótico humano com TGF-ß3 em cultura de esferóides cultivados em hidrogel termosensível para regeneração de lesões de cartilagem |
| Beneficiário: | Ibsen Bellini Coimbra |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |