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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Copia Retrotransposon in the Zaprionus Genus: Another Case of Transposable Element Sharing with the Drosophila melanogaster Subgroup

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Autor(es):
de Setta, Nathalia [1, 2] ; Van Sluys, Marie-Anne [2] ; Capy, Pierre [3, 4] ; Aparecida Carareto, Claudia Marcia [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, UNESP, Dept Biol, Lab Mol Evolut, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Bot, Genom & Transposable Elements Lab, BR-05508090 Sao Paulo - Brazil
[3] CNRS, UPR9034, Lab Evolut Genomes & Speciat, F-91198 Gif Sur Yvette - France
[4] Univ Paris 11, F-91405 Orsay - France
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Journal of Molecular Evolution; v. 72, n. 3, p. 326-338, MAR 2011.
Citações Web of Science: 4
Resumo

Copia is a retrotransposon that appears to be distributed widely among the Drosophilidae subfamily. Evolutionary analyses of regulatory regions have indicated that the Copia retrotransposon evolved through both positive and purifying selection, and that horizontal transfer (HT) could also explain its patchy distribution of the among the subfamilies of the melanogaster subgroup. Additionally, Copia elements could also have transferred between melanogaster subgroup and other species of Drosophilidae-D. willistoni and Z. tuberculatus. In this study, we surveyed seven species of the Zaprionus genus by sequencing the LTR-ULR and reverse transcriptase regions, and by using RT-PCR in order to understand the distribution and evolutionary history of Copia in the Zaprionus genus. The Copia element was detected, and was transcriptionally active, in all species investigated. Structural and selection analysis revealed Zaprionus elements to be closely related to the most ancient subfamily of the melanogaster subgroup, and they seem to be evolving mainly under relaxed purifying selection. Taken together, these results allowed us to classify the Zaprionus sequences as a new subfamily-ZapCopia, a member of the Copia retrotransposon family of the melanogaster subgroup. These findings indicate that the Copia retrotransposon is an ancient component of the genomes of the Zaprionus species and broaden our understanding of the diversity of retrotransposons in the Zaprionus genus. (AU)

Processo FAPESP: 04/02851-9 - Elementos de transposição e seu impacto no genoma de bactérias e plantas
Beneficiário:Marie-Anne van Sluys
Linha de fomento: Auxílio à Pesquisa - Temático