Prevalence of virulence factors in Escherichia coli isolated from healthy animals and water sources in Brazil

Autor(es)

Carlos, Camila ^[1] ; Alexandrino, Fabiana ^[1] ; Vieira, Monica A. M. ^[2] ; Stoppe, Nancy C. ^[1] ; Sato, Maria Ines Z. ^[3] ; Gomes, Tania A. T. ^[2] ; Ottoboni, Laura M. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas UNICAMP, Ctr Biol Mol & Engn Genet, BR-13083875 Campinas, SP - Brazil

^[2] Univ Fed Sao Paulo UNIFESP, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo - Brazil

^[3] Co Ambiental Estado Sao Paulo CETESB, Dept Anal Ambientais, BR-05459900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF WATER AND HEALTH; v. 9, n. 1, p. 138-142, MAR 2011.
• Citações Web of Science: 4

Resumo

The aim of this work was to verify the presence of seven virulence factors (ST. LT, eae, stx(1), stx(2), INV and EAEC) among Escherichia coli strains isolated from healthy humans, bovines, chickens, sheep, pigs and goats, from two sewage treatment plants and from the Tiete River. We have found a high prevalence of eae, stx(1) and stx(2) in ruminants. The EAEC gene was only found in humans and sewage. No strains presented ST, LT or INV. BOX-PCR fingerprints revealed a high diversity among the strains analysed and a non-clonal origin of strains that presented the same virulence factors. Therefore, we concluded that ruminants may constitute an important reservoir of most diarrheagenic E. coli in Brazil, except for EAEC strains. These results emphasize the importance of the identification of the animal source of fecal contamination for the correct water risk assessment. (AU)

• Processo FAPESP: 07/57025-4 - Identificação de marcadores moleculares hospedeiro-especificos de Escherichia coli de águas superficiais do estado de São Paulo
• Beneficiário: Camila Carlos
• Modalidade de apoio: Bolsas no Brasil - Mestrado