| Texto completo | |
| Autor(es): Mostrar menos - |
de Barros, Amanda Vieira
;
de Veras, Bruno Oliveira
;
Menezes, Gabriela de Lima
;
Bezerra, Katyanna Sales
;
dos Santos, Patryck Ermerson Monteiro
;
de Sa, Rafael Artur De Queiroz Cavalcanti
;
Fulco, Umberto Laino
;
Galvao, Douglas Soares
;
Paiva, Patricia Maria Guedes
;
Malafaia, Guilherme
;
da Silva, Marcia Vanusa
;
Coutinho, Henrique Douglas Melo
;
de Oliveira, Maria Betania Melo
Número total de Autores: 13
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Microbial Pathogenesis; v. 210, p. 13-pg., 2025-10-27. |
| Resumo | |
The escalating antimicrobial resistance of Klebsiella pneumoniae poses a critical public health challenge, demanding innovative therapeutic strategies. This study investigated the antibacterial activity of eugenol (EOL) and its potential as a resistance-modulating agent when combined with conventional antibiotics-amoxicillin (AXL), azithromycin (AZT), cephalexin (CEF), and ciprofloxacin (CIP)-against clinical multidrug-resistant isolates. EOL exhibited intrinsic antibacterial activity with MIC values ranging from 1024 to 2048 mu g/mL. Checkerboard assays revealed synergistic interactions between EOL and AXL or AZT (FICI <= 0.5), while combinations with CEF and CIP were indifferent. These synergistic effects were corroborated by growth inhibition curves, time-kill kinetics, and biofilm suppression assays, all of which demonstrated a marked reduction in bacterial viability and biofilm formation. Molecular docking and quantum mechanical calculations further elucidated the enhanced binding affinities and intermolecular interactions between AXL-EOL complexes and key resistance-related targets (KPC, LpxC, and particularly the quorum-sensing regulator SdiA), with interaction energies reaching up to-52.06 kcal/mol. Altogether, the findings underscore the potential of EOL as a potent adjuvant that augments the efficacy of conventional antibiotics, offering a promising pathway toward the development of targeted therapies against multidrug-resistant K. pneumoniae. (AU) | |
| Processo FAPESP: | 13/08293-7 - CECC - Centro de Engenharia e Ciências Computacionais |
| Beneficiário: | Munir Salomao Skaf |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |