Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Immunohistochemical and molecular expression of laminin-332 gamma-2 chain in canine mammary tumors

Texto completo
Autor(es):
Zuccari, D. A. P. C. [1] ; Castro, R. [1] ; Jardim, B. V. [1, 2] ; Mancini, U. M. [1] ; Polachini, G. M. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Fac Med Sao Jose do Rio Preto, BR-5090000 Sao Jose Do Rio Preto, SP - Brazil
[2] UNESP, IBILCE, Sao Jose Do Rio Preto, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Arquivo Brasileiro de Medicina Veterinária e Zootecnia; v. 63, n. 1, p. 28-35, FEB 2011.
Citações Web of Science: 0
Resumo

Forty-eight cases of canine mammary cancer were investigated to evaluate the immunohistochemical distribution of the gamma 2 chain of laminin-332. Tumor cells were compared to a pool of normal mammary tissues using quantitative RT-PCR. The western blot was performed in eight tumor samples as complementary test to evaluate protein integrity. Immunohistochemistry experiments showed negative, focal, and weak expression of laminin-332 gamma 2 in tumors with the worst prognosis. Quantitative PCR revealed downregulation of the gene in 27 (56.2%) of the animals. Out of the 16 dogs with gamma 2 chain overexpression, seven were still alive. The western blot results showed bands generation of 36, 50, and 98kDa, suggesting degradation of laminin-332 gamma 2 in malignant tumors. The results suggest that, in the future, low expression and/or degradation of laminin-332 gamma 2 chain in canine mammary tumors may be used as an indicator of malignant potential. However, further studies are necessary to corroborate these results. (AU)

Processo FAPESP: 05/51936-0 - Avaliação imuno-histoquímica e molecular dos genes caveolina-1, interleucina-8, laminina-5 e serpin como marcadores prognósticos e preditivos nas neoplasias mamárias em cadelas
Beneficiário:Debora Aparecida Pires de Campos Zuccari
Linha de fomento: Auxílio à Pesquisa - Regular