The non-coding RNA BC1 is down-regulated in the hippocampus of Wistar Audiogenic Rat (WAR) strain after audiogenic kindling

Autor(es)

Goes Gitai, Daniel Leite ^[1] ; Fachin, Ana Lucia ^[2] ; Mello, Stephano Spano ^[2] ; Elias, Carol Fuzachi ^[3] ; Bittencourt, Jackson Cioni ^[3] ; Leite, Joao Pereira ^[4] ; da Silva Passos, Geraldo Aleixo ^[2] ; Garcia-Cairasco, Norberto ^[5] ; Paco-Larson, Maria Luisa ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Cellular & Mol Biol, BR-05508 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Genet, Mol Immunogenet Grp, BR-05508 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, BR-05508 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Neurosci & Behav, BR-05508 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Physiol, BR-05508 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brain Research; v. 1367, p. 114-121, JAN 7 2011.
• Citações Web of Science: 13

Resumo

The aim of this study was to identify molecular pathways involved in audiogenic seizures in the epilepsy-prone Wistar Audiogenic Rat (WAR). For this, we used a suppression-subtractive hybridization (SSH) library from the hippocampus of WARs coupled to microarray comparative gene expression analysis, followed by Northern blot validation of individual genes. We discovered that the levels of the non-protein coding (npc) RNA BC1 were significantly reduced in the hippocampus of WARs submitted to repeated audiogenic seizures (audiogenic kindling) when compared to Wistar resistant rats and to both naive WARs and Wistars. By quantitative in situ hybridization, we verified lower levels of BC1 RNA in the GD-hilus and significant signal ratio reduction in the stratum radiatum and stratum pyramidale of hippocampal CA3 subfield of audiogenic kindled animals. Functional results recently obtained in a BC1-/- mouse model and our current data are supportive of a potential disruption in signaling pathways, upstream of BC1, associated with the seizure susceptibility of WARs. (C) 2010 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 07/50261-4 - Neurociências, epilepsia e arte: caracterização interdisciplinar da cepa WAR e modelos de epilepsia correlacionados: as oficinas Da Vinci, divulgação científica e quebras de estigmas em epilepsia
• Beneficiário: Norberto Garcia Cairasco
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 02/13828-2 - Análise da expressão gênica diferencial no hipocampo de pacientes com epilepsia de lobo temporal
• Beneficiário: Daniel Leite Góes Gitaí
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 05/56447-7 - Pesquisa através de imagens por ressonância magnética de alto campo destinadas a estudos em humanos
• Beneficiário: João Pereira Leite
• Linha de fomento: Auxílio à Pesquisa - Programa CINAPCE - Temático