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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Dendritic Cells Transfected with scFv from Mab 7.B12 Mimicking Original Antigen gp43 Induces Protection against Experimental Paracoccidioidomycosis

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Autor(es):
Ferreira, Karen S. [1] ; Maranhao, Andrea Q. [2] ; Garcia, Maria C. C. [3] ; Brigido, Marcelo M. [2] ; Santos, Suelen S. [3] ; Lopes, Jose D. [4] ; Almeida, Sandro R. [3]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Dept Ciencias Biol, Sao Paulo - Brazil
[2] Univ Brasilia, Dept Biol Celular, Brasilia, DF - Brazil
[3] Univ Sao Paulo, Dept Anal Clin & Toxicol, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 6, n. 1 JAN 7 2011.
Citações Web of Science: 7
Resumo

Paracoccidioidomycosis (PCM), endemic in Latin America, is a progressive systemic mycosis caused by Paracoccidioides brasiliensis (P. brasiliensis), which primarily attacks lung tissue. Dendritic cells (DCs) are able to initiate a response in naive T cells, and they also participate in Th-cell education. Furthermore, these cells have been used for therapy in several disease models. Here we transfected DCs with a plasmid (pMAC/PS-scFv) encoding a single chain variable fragment (scFv) of an anti-Id antibody that is capable of mimicking gp43, the main antigenic component of P. brasiliensis. First, Balb/c mice were immunized subcutaneously with pMAC/PS-scFv and, after seven days, scFv protein was presented to the regional lymph nodes cells. Moreover, we showed that the DCs transfected with scFv were capable of efficiently activating proliferation of total lymph node cells and inducing a decrease in lung infection. Therefore, our results suggested that the use of scFv-transfected DCs may be a promising therapy in the paracoccidioidomycosis (PCM) model. (AU)

Processo FAPESP: 07/50568-2 - Produção de scFv a partir de anticorpo monoclonal anti-idiotípico Ab2-beta, quemimetiza o antígeno gp43 de Paracoccidioides Brasiliensis
Beneficiário:Karen Spadari Ferreira
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 07/57594-9 - Produção de sc-fv a partir de anticorpo monocional anti-idiotípico ab2beta que mimetiza o antígeno gp43 de Paracoccidioides Brasiliensis
Beneficiário:Sandro Rogerio de Almeida
Linha de fomento: Auxílio à Pesquisa - Regular