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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Vitamin B-12 Supplement Exerts a Beneficial Effect on the Seminiferous Epithelium of Cimetidine-Treated Rats

Texto completo
Autor(es):
Beltrame, Flavia L. [1] ; Caneguim, Breno H. [1] ; Miraglia, Sandra M. [1] ; Cerri, Paulo S. [2] ; Sasso-Cerri, Estela [2]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Fed Univ Sao Paulo Unifesp, Dept Morphol & Genet, Sao Paulo - Brazil
[2] Sao Paulo State Univ UNESP, Dept Morphol, Lab Histol & Embryol, Sch Dent, Araraquara - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Cells Tissues Organs; v. 193, n. 3, p. 184-194, 2011.
Citações Web of Science: 12
Resumo

Treatment of gastric ulcer with cimetidine reduces acid secretion and interferes in the vitamin B(12) absorption. Regarding the harmful effect of cimetidine on the seminiferous tubules, the aim of the present study was to verify if prolonged treatment with cimetidine causes vitamin B(12) deficiency and whether the testicular damages are attenuated by vitamin B(12) supplementation. Adult male rats received, for 50 days, cimetidine (CMTG), cimetidine and vitamin B(12) (CMT/B(12)G), vitamin B(12) (B(12)G) and saline solution (CG). Vitamin B(12) and homocysteine plasma levels were evaluated and the testes were embedded in glycol methacrylate for the morphometric analyses of total, epithelial and luminal areas of the seminiferous tubules, number of Sertoli cells and frequencies of tubules according to stages and containing Sertoli and germ cells in the lumen. Terminal deoxynucleotidyl-transferase mediated dUTP nick end labeling (TUNEL) method and proliferating cell nuclear antigen (PCNA) immunohistochemistry were carried out. CMTG showed TUNEL-positive Sertoli cells and significant reductions in the epithelial and total tu-bular areas, number of Sertoli cells and frequency of tubules VII-VIII. In the CMT/B(12)G, the number of Sertoli cells and the epithelial and total tubular areas were similar to CG. The number of Sertoli cells (in B(12)G) and the frequency of tubules at stages VII-VIII (in B(12)G and CMT/B(12)G) increased significantly; PCNA-positive Sertoli cells were found in these groups. Although cimetidine was not able to induce vitamin B(12) deficiency, this drug causes tubular atrophy due to Sertoli cell damage and loss of germ cells. However, vitamin B(12) supplement is able to stimulate spermatogenesis and restore the number of Sertoli cells, softening the harmful effect of cimetidine on spermatogenesis. Copyright (C) 2010 S. Karger AG, Basel (AU)

Processo FAPESP: 06/54776-6 - Avaliação morfológica e morfométrica de testículos de ratos tratados com cimetidina, com ênfase na célula de Sertoli e na vascularização intersticial
Beneficiário:Estela Sasso Cerri
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 07/53207-0 - Avaliação morfológica e morfométrica dos testículos de ratos suplementados com Vitamina B12 durante o tratamento com cimetidina
Beneficiário:Flávia Luciana Beltrame
Linha de fomento: Bolsas no Brasil - Mestrado