Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Extractive Fermentation of Clavulanic Acid by Streptomyces DAUFPE 3060 Using Aqueous Two-Phase System

Texto completo
Autor(es):
Viana Marques, D. A. [1] ; Pessoa-Junior, A. [1] ; Lima-Filho, J. L. [2] ; Converti, A. [3] ; Perego, P. [3] ; Porto, A. L. F. [4]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Pharmaceut Technol & Biochem, BR-05508000 Sao Paulo - Brazil
[2] Univ Fed Pernambuco, Dept Biochem, BR-50670901 Recife, PE - Brazil
[3] Univ Genoa, Dept Chem & Proc Engn, I-16145 Genoa - Italy
[4] Univ Fed Rural Pernambuco, Dept Anim Morphol & Physiol, BR-52171900 Dois Irmaos, PE - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: BIOTECHNOLOGY PROGRESS; v. 27, n. 1, p. 95-103, JAN-FEB 2011.
Citações Web of Science: 16
Resumo

The influence of four variables, specifically PEG molar mass (400, 1,000, and 8,000 g/mol), concentrations of PEG and phosphate salts (15, 20, and 25% for both), and agitation intensity (110, 150, and 200 rpm), on clavulanic acid (CA) extraction by extractive fermentation with PEG/phosphate salts aqueous two-phase system was investigated in shaken flasks using a 2(4-1)-fractional factorial design. After selection of the two most significant variables (agitation intensity and PEG molar mass), an optimization study conducted according to a 2(2)-central composite design revealed that 25% PEG 8,000 g/mol and phosphate salts at 240 rpm (run 6) were the best conditions for the extractive fermentation, leading to the best results in terms of partition coefficient (k = 8.2), yield of CA in the PEG-rich phase (eta(T) = 93%) and productivity (P = 5.3 mg/Lh). As a first attempt to make a scale-up of these results, the effectiveness of the extractive fermentation was then checked in a bench-scale bioreactor under conditions as close as possible to the optimum ones determined in flasks. The highest CA concentration obtained in the PEG-rich phase (691 mg/L) was 30% higher than in flasks, thus demonstrating the potential of such a new process, integrating the production and extraction steps, as a promising, low-cost tool to obtain high yields of this and similar products. (C) 2010 American Institute of Chemical Engineers Biotechnol. Prog., 27: 95-103, 2011 (AU)

Processo FAPESP: 05/60158-0 - Produção e extração de ácido clavulânico de Streptomyces spp. por fermentação extrativa utilizando sistemas de duas fases aquosas
Beneficiário:Daniela de Araújo Viana Marques
Linha de fomento: Bolsas no Brasil - Doutorado