Quantitative Detection of Enterotoxigenic Bacteroides fragilis Subtypes Isolated from Children with and without Diarrhea

Autor(es)

Merino, V. R. C. ^[1] ; Nakano, V. ^[1] ; Liu, C. ^[2] ; Song, Y. ^[2] ; Finegold, S. M. ^{[2, 3, 4]} ; Avila-Campos, M. J. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Anaerobe Lab, Dept Microbiol, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil

^[2] VA Med Ctr W Los Angeles, Los Angeles, CA - USA

^[3] UCLA Sch Med, Dept Med, Los Angeles, CA - USA

^[4] UCLA Sch Med, Dept Microbiol Mol Genet & Immunol, Los Angeles, CA - USA

• Tipo de documento: Artigo Científico
• Fonte: Journal of Clinical Microbiology; v. 49, n. 1, p. 416-418, JAN 2011.
• Citações Web of Science: 13

Resumo

A rapid real-time PCR ( RT-PCR) approach was developed to detect the bft gene subtypes in Bacteroides fragilis isolated from fecal samples. DNA obtained from diarrhea (110) and nondiarrhea (150) samples was evaluated. Subtype 1 was observed in 9 (8.2%) diarrhea and 7 (4.7%) nondiarrhea samples. Subtype 2 was not detected in any DNA samples, and subtype 3 was observed in only 1 diarrhea sample. The presence of the bft-1 gene did not show any statistically significant differences between the groups of children. This technique could be used to evaluate a possible correlation between disease and the presence of B. fragilis enterotoxin. (AU)

• Processo FAPESP: 07/03655-7 - Avaliação quantitativa de cepas enterotoxigênicas de Bacteroides fragilis e dos respectivos subtipos do gene BFT em crianças imunocompetentes com e sem diarreia aguda
• Beneficiário: Victor Rafael Castillo Merino
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto