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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Characterization of Hepatitis B virus (HBV) genotypes in patients from Rondonia, Brazil

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Autor(es):
Santos, Alcione O. [1] ; Alvarado-Mora, Monica V. [2, 3] ; Botelho, Livia [2, 3] ; Vieira, Deusilene S. [1] ; Rebello Pinho, Joao R. [2, 3] ; Carrilho, Flair J. [2, 3] ; Honda, Eduardo R. [1] ; Salcedo, Juan M. [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] CEPEM Trop Pathol Res Inst IPEPATRO, Res Ctr Trop Med, Porto Velho, RO - Brazil
[2] Univ Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo - Brazil
[3] Univ Sao Paulo, Lab Gastroenterol & Hepatol, Sao Paulo Inst Trop Med, Sch Med, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: VIROLOGY JOURNAL; v. 7, NOV 12 2010.
Citações Web of Science: 39
Resumo

Background: Hepatitis B virus (HBV) can be classified into nine genotypes (A-I) defined by sequence divergence of more than 8% based on the complete genome. This study aims to identify the genotypic distribution of HBV in 40 HBsAg-positive patients from Rondonia, Brazil. A fragment of 1306 bp partially comprising surface and polymerase overlapping genes was amplified by PCR. Amplified DNA was purified and sequenced. Amplified DNA was purified and sequenced on an ABI PRISM (R) 377 Automatic Sequencer (Applied Biosystems, Foster City, CA, USA). The obtained sequences were aligned with reference sequences obtained from the GenBank using Clustal X software and then edited with Se-Al software. Phylogenetic analyses were conducted by the Markov Chain Monte Carlo (MCMC) approach using BEAST v.1.5.3. Results: The subgenotypes distribution was A1 (37.1%), D3 (22.8%), F2a (20.0%), D4 (17.1%) and D2 (2.8%). Conclusions: These results for the first HBV genotypic characterization in Rondonia state are consistent with other studies in Brazil, showing the presence of several HBV genotypes that reflects the mixed origin of the population, involving descendants from Native Americans, Europeans, and Africans. (AU)

Processo FAPESP: 07/53457-7 - Estudo da variabilidade genotípica dos vírus das Hepatites B e C na Colômbia
Beneficiário:João Renato Rebello Pinho
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/50461-6 - Estudo da variabilidade genotípica dos vírus da Hepatite B e Hepatite C na Colômbia
Beneficiário:Monica Viviana Alvarado Mora
Linha de fomento: Bolsas no Brasil - Doutorado Direto