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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Interleukin-15 augments oxidative metabolism and fungicidal activity of human monocytes against Paracoccidioides brasiliensis

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Autor(es):
Bannwart, Camila Ferreira [1] ; Rodrigues Martins, Rosana A. [1] ; Nakaira-Takahashi, Erika [1] ; Dias-Melicio, Luciane A. [1] ; Campos Soares, Angela M. V. [1] ; Peracoli, Maria Terezinha S. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, Dept Microbiol & Imunol, Inst Biociencias, Botucatu, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Memórias do Instituto Oswaldo Cruz; v. 105, n. 7, p. 866-872, NOV 2010.
Citações Web of Science: 4
Resumo

Interleukin (IL)-15 is a pleiotropic cytokine that regulates the proliferation and survival of many cell types. IL-15 is produced by monocytes and macrophages against infectious agents and plays a pivotal role in innate and adaptive immune responses. This study analyzed the effect of IL-15 on fungicidal activity, oxidative metabolism and cytokine production by human monocytes challenged in vitro with Paracoccidioides brasiliensis (Pb18), the agent of paracoccidioidomycosis. Peripheral blood monocytes were pre-incubated with IL-15 and then challenged with Pb18. Fungicidal activity was assessed by viable fungi recovery from cultures after plating on brain-heart infusion-agar. Superoxide anion (O(2)(-)), hydrogen peroxide (H(2)O(2)), tumour necrosis factor-alpha (TNF-alpha), IL-6, IL-15 and IL-10 production by monocytes were also determined. IL-15 enhanced fungicidal activity against Pb18 in a dose-dependent pattern. This effect was abrogated by addition of anti-IL-15 monoclonal antibody. A significant stimulatory effect of IL-15 on O(2)(-) and H(2)O(2) release suggests that fungicidal activity was dependent on the activation of oxidative metabolism. Pretreatment of monocytes with IL-15 induced significantly higher levels of TNF-alpha, IL-10 and IL-15 production by cells challenged with the fungus. These results suggest a modulatory effect of IL-15 on pro and anti-inflammatory cytokine production, oxidative metabolism and fungicidal activity of monocytes during Pb18 infection. (AU)

Processo FAPESP: 05/54283-7 - Atividade fungicida de monócitos humanos infectados in vitro com Paracoccidioides Brasiliensis: atividade fungicida, metabolismo oxidativo e produção de citocinas
Beneficiário:Camila Ferreira Bannwart Castro
Linha de fomento: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
Processo FAPESP: 03/13743-0 - Atividade funcional de monócitos humanos infectados in vitro com Paracoccidioides Brasiliensis: atividade fungicida, metabolismo oxidativo e produção de citocinas
Beneficiário:Maria Terezinha Serrão Peraçoli
Linha de fomento: Auxílio à Pesquisa - Regular