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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Ex vivo assessment of genotoxicity and cytotoxicity in murine fibroblasts exposed to white MTA or white Portland cement with 15% bismuth oxide

Texto completo
Autor(es):
Zeferino, E. G. [1] ; Bueno, C. E. S. [1] ; Oyama, L. M. [2] ; Ribeiro, D. A. [2]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Sao Leopoldo Mand Univ, Dent Res Ctr, Endodont Area, Campinas, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Biociencias, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: International Endodontic Journal; v. 43, n. 10, p. 843-848, OCT 2010.
Citações Web of Science: 19
Resumo

P>Aims To evaluate whether white mineral trioxide aggregate (MTA) or white Portland cement with 15% bismuth oxide were able to induce genetic damage and cellular death ex vivo. Methodology Aliquots of 1 x 104 murine fibroblasts were incubated at 37 degrees C for 3 h with MTA (white) or white Portland cement with 15% bismuth oxide, at final concentrations ranging from 10 to 1000 mu g mL-1 individually. Data of three independent repeats from the comet assay and the trypan blue exclusion test were assessed by the one-way anova followed by Tukey's test. Results Mineral trioxide aggregate or Portland cement containing bismuth oxide did not produce genotoxic effects with respect to the single-cell gel (comet) assay data for all concentrations evaluated. Furthermore, no cytotoxicity was observed for MTA or Portland cement. Conclusion White MTA or white Portland cement containing 15% bismuth oxide were not genotoxic and cytotoxic. (AU)

Processo FAPESP: 07/01228-4 - Carcinogênese bucal quimicamente induzida pela 4-nitroquinolina 1-óxido em ratos: possíveis biomarcadores envolvidos em sua patogênese
Beneficiário:Daniel Araki Ribeiro
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores