Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Transient disruption of liver gap junctional intercellular communication and induction of apoptosis after administration of 1,4-bis[2-(3,5 dichloropyridyloxy)]benzene in mice

Texto completo
Autor(es):
Fukumasu, Heidge [1] ; Sanches, Daniel S. [1] ; da Silva, Tereza C. [1] ; Ward, Jerrold M. [2] ; Dagli, Maria L. Z. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Lab Expt & Comparat Oncol, Sch Vet Med & Anim Sci, Sao Paulo - Brazil
[2] Global Vet Pathol, Montgomery Village, MD 20886 - USA
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY; v. 62, n. 5, p. 525-531, SEP 2010.
Citações Web of Science: 3
Resumo

Gap junctional intercellular communication (GJIC) and connexin expression (Cx26 and Cx32) in mouse liver were studied after administration of 4-bis{[}2-(3,5 dichloropyridyloxy)]benzene (TCPOBOP), a phenobarbital-like enzyme inducer. Female C57BI/6 mice were administered TCPOBOP (5.8 mg/kg BW) and euthanized 0, 24, 48 and 72 hours later. Liver samples were snap frozen, or fixed in formalin, or submitted to GJIC analysis. The proliferating cell nuclear antigen (PCNA) immunohistochemistry and the Western blotting for Cx26 and Cx32 were performed. After 48 and 72 h of drug administration the liver-to-body weight ratio was increased 70% and 117% (p < 0.0001), respectively. There were temporal-dependent alterations in liver histopathology and a significant increase in cell proliferation was noted after 48h and sustained after 72h, though to a lesser extent (p < 0.0001). In addition. TCPOBOP administration induced apoptosis, which appeared to be time-dependent showing statistical significance only after 72h (p < 0.0001). Interestingly, a transient disruption by nearly 50% of GJIC capacity was detected after 48 h of drug ingestion, which recovered after 72 h (p = 0.003). These GJIC changes were due to altered levels of Cx26 and Cx32 in the livers of TCPOBOP-treated mice. We concluded that a single administration of TCPOBOP transiently disrupted the levels of GJIC due to decreased expression of connexins and increased apoptotic cell death in mouse liver. (C) 2009 Elsevier GmbH. All rights reserved. (AU)

Processo FAPESP: 05/54194-4 - Avaliação da ação quimiopreventiva do guaraná (Paullinia cupana Mart. var. sorbilis) em camundongos com deficiência na expressão da Cx43 submetidos à carcinogênese pulmonar induzida por carcinógeno do tabaco, NNK
Beneficiário:Heidge Fukumasu
Linha de fomento: Bolsas no Brasil - Doutorado Direto