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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Liposomes incorporating essential oil of Brazilian cherry (Eugenia uniflora L.): Characterization of aqueous dispersions and lyophilized formulations

Texto completo
Autor(es):
Yoshida, P. A. [1] ; Yokota, D. [1] ; Foglio, M. A. [2] ; Rodrigues, R. A. F. [2] ; Pinho, S. C. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] USP, Sch Anim Sci & Food Engn FZEA, Dept Food Engn, BR-13635900 Pirassununga, SP - Brazil
[2] Univ Estadual Campinas, Div Phytochem, Pluridisciplinar Ctr Biol Chem & Agr Res CPQBA, Campinas, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Journal of Microencapsulation; v. 27, n. 5, p. 416-425, AUG 10 2010.
Citações Web of Science: 28
Resumo

Multilamellar liposomes incorporating essential oil of Brazilian cherry (Eugenia uniflora L.) leaves were produced by dry film hydration. Gas chromatography demonstrated the compounds found in the essential oil were effectively incorporated in the aqueous dispersions of liposomes. Differential scanning calorimetry analyses revealed the incorporation of the essential oil did not cause phase separation in the membrane structure; the gel-liquid crystalline transition temperature (main transition) remained the same despite the higher heterogeneity indicated by the transition peak broadening. Different cryoprotectors (sucrose and trehalose) were added to the liposomal formulations to be tested in their ability to protect the liposomal structure during the lyophilization. The morphological aspect of the lyophilized powders analysed by scanning electron microscopy showed significant differences among the samples with and without cryoprotectors. Fourier-transform infrared spectroscopy indicated the cryoprotectors interacted effectively with the polar heads of phospholipids in the bilayer. In terms of water absorption, trehalose was identified as a much more effective protector agent against it than sucrose. The cryoprotectors showed different degrees of effectiveness of preservation of the liposomal structure when the rehydration assays of lyophilized liposomes were carried out, as particle size measurements indicated a moderate process of fusion when the formulations with sucrose were rehydrated. (AU)

Processo FAPESP: 06/00178-0 - Estudo da viabilidade de microencapsulação de óleo essencial de fruta tropical (Eugenia uniflora L.) em lipossomas
Beneficiário:Samantha Cristina de Pinho
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 06/02471-7 - Viabilidade de microencapsulação de óleo essencial de pitanga (Eugenia uniflora L.) em lipossomas para aplicação em alimentos
Beneficiário:Patrícia Akemi Yoshida
Linha de fomento: Bolsas no Brasil - Iniciação Científica