Profiling the Proteome of the Venom from the Social Wasp Polybia paulista: A Clue to Understand the Envenoming Mechanism

Autor(es):Mostrar menos -

dos Santos, Lucilene Delazari ^[1] ; Santos, Keity Souza ^[2] ; Aparecido Pinto, Jose Roberto ^[1] ; Dias, Nathalia Baptista ^[1] ; de Souza, Bibiana Monson ^[1] ; dos Santos, Manse Fonseca ^[3] ; Perales, Jonas ^[4] ; Domont, Gilberto Barbosa ^[3] ; Castro, Fabio Morato ^[2] ; Kalil, Jorge Elias ^[2] ; Palma, Mario Sergio ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo State UNESP, Ctr Study Social Insects, Dept Biol, Inst Biosci Rio Claro, Rio Claro, SP - Brazil

^[2] Discipline Allergy & Immunol InCor HC FMUSP, Sao Paulo - Brazil

^[3] Univ Fed Rio de Janeiro, Dept Biochem, BR-21941 Rio De Janeiro - Brazil

^[4] Fiocruz MS, Dept Physiol & Pharmacodynam, Rio De Janeiro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROTEOME RESEARCH; v. 9, n. 8, p. 3867-3877, AUG 2010.
• Citações Web of Science: 44

Resumo

The study reported here is a classical bottom-up proteomic approach where proteins from wasp venom were extracted and separated by 2-DE; the individual protein spots were proteolytically digested and subsequently identified by using tandem mass spectrometry and database query with the protein search engine MASCOT. Eighty-four venom proteins belonging to 12 different molecular functions were identified. These proteins were classified into three groups; the first is constituted of typical venom proteins: antigens-5, hyaluronidases, phospholipases, heat shock proteins, metalloproteinases, metalloproteinase-desintegrin like proteins, serine proteinases, proteinase inhibitors, vascular endothelial growth factor-related protein, arginine kinases, Sol i-II and -II like proteins, alpha-glucosidase, and superoxide dismutases. The second contained proteins structurally related to the muscles that involves the venom reservoir. The third group, associated with the housekeeping of cells from venom glands, was composed of enzymes, membrane proteins of different types, and transcriptional factors. The composition of P. paulista venom permits us to hypothesize about a general envenoming mechanism based on five actions: (i) diffusion of venom through the tissues and to the blood, (ii) tissue, (iii) hemolysis, (iv) inflammation, and (v) allergy-played by antigen-5, PLA1, hyaluronidase, HSP 60, HSP 90, and arginine kinases. (AU)

• Processo FAPESP: 06/57122-7 - Procura de compostos líderes para o desenvolvimento racional de novos fármacos e pesticidas a partir bioprospecção da fauna de artrópodes brasileiros
• Beneficiário: Mario Sergio Palma
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático