Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Neutral Aminopeptidase and Dipeptidyl Peptidase IV Activities in Plasma of Monosodium Glutamate Obese and Food-deprived Rats

Texto completo
Autor(es):
Alponti, Rafaela F. [1, 2] ; Silveira, Paulo F. [1]
Número total de Autores: 2
Afiliação do(s) autor(es):
[1] Inst Butantan, Pharmacol Lab, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Neurosci & Behav, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: OBESITY; v. 18, n. 7, p. 1312-1317, JUL 2010.
Citações Web of Science: 9
Resumo

Biometric parameters, glycemia and activity levels of plasma neutral aminopeptidase (APN) and dipeptidyl peptidase IV (DPPIV) were measured in monosodium glutamate obese and food-deprived rats (MSG-FD), to analyze the involvement of these enzymes in such situations. Plasma APN was distinguished as sensitive (PSA) (K(m) = 7.8 x 10(-5) mol/l) and predominantly insensitive (APM) (K(m) = 21.6 x 10(-5) mol/l) to puromycin, whereas DPPIV was sensitive (DPPIV-DS) (K(m) = 0.24 x 10(-5) mol/l) and predominantly insensitive (DPPIV-DI) (K(m) = 7.04 x 10(-5) mol/l) to diprotin A. Although unchanged in the MSG and food-deprived animals, APM activity levels were closely correlated with body mass, Lee index, and mass of retroperitoneal fat pad in the food deprived, but not in the MSG animals. DPPIV-DI activity levels decreased by 33% and were correlated with body mass, Lee index, and mass of periepididymal fat pad in the food-deprived MSG rats. These data suggest that APM and DPPIV-DI are respectively related to the downregulation of somatostatin in food-deprived rats, and to the recovery of energy balance in MSG obese rats during food deprivation. (AU)

Processo FAPESP: 05/04699-2 - Aminopeptidase neutra (APN/CD13), prolil dipeptidil aminopeptidase IV (DPPIV/CD26) e C-FOS no hipotálamo e hipocampo de ratos privados de alimento e com obesidade induzida por glutamato monosódico
Beneficiário:Paulo Flávio Silveira
Linha de fomento: Auxílio à Pesquisa - Regular