| Texto completo | |
| Autor(es): |
Estofolete, Cassia F.
[1]
;
Botelho-Machado, Carla
[1]
;
Taboga, Sebastiao R.
[2]
;
Zucoloto, Sergio
[3]
;
Polli-Lopes, Ana Claudia
[1]
;
Gil, Cristiane D.
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] FAMERP, Sch Med, Dept Anat, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil
[2] UNESP, IBILCE, Inst Biosci Humanities & Exact Sci, Dept Biol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[3] Univ Sao Paulo, FMRP, Sch Med, Dept Pathol, BR-14049900 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CANCER CELL INTERNATIONAL; v. 10, JUN 22 2010. |
| Citações Web of Science: | 2 |
| Resumo | |
Background: In this study the effect of myenteric denervation induced by benzalconium chloride (BAC) on distribution of fibrillar components of extracellular matrix (ECM) and inflammatory cells was investigated in gastric carcinogenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Rats were divided in four experimental groups: non-denervated (I) and denervated stomach (II) without MNNG treatment; non-denervated (III) and denervated stomachs (IV) treated with MNNG. For histopathological, histochemical and stereological analysis, sections of gastric fragments were stained with Hematoxylin-Eosin, Picrosirius-Hematoxylin, Gomori reticulin, Weigert's Resorcin-Fuchsin, Toluidine Blue and Alcian-Blue/Safranin (AB-SAF). Results: BAC denervation causes an increase in the frequency of reticular and elastic fibers in the denervated (group II) compared to the non-denervated stomachs (group I). The treatment of the animals with MNNG induced the development of adenocarcinomas in non-denervated and denervated stomachs (groups III and IV, respectively) with a notable increase in the relative volume of the stroma, the frequency of reticular fibers and the inflammatory infiltrate that was more intense in group IV. An increase in the frequency of elastic fibers was observed in adenocarcinomas of denervated (group IV) compared to the non-denervated stomachs (group III) that showed degradation of these fibers. The development of lesions (groups III and IV) was also associated with an increase in the mast cell population, especially AB and AB-SAF positives, the latter mainly in the denervated group IV. Conclusions: The results show a strong association in the morphological alteration of the ECM fibrillar components, the increased density of mast cells and the development of tumors induced by MNNG in the non-denervated rat stomach or denervated by BAC. This suggests that the study of extracellular and intracellular components of tumor microenvironment contributes to understanding of tumor biology by action of myenteric denervation. (AU) | |
| Processo FAPESP: | 08/05722-6 - Estudo da expressão das proteínas galectina-3 e anexina-A1 nas células inflamatórias em modelo experimental de tumorigênese gástrica em ratos |
| Beneficiário: | Cássia Fernanda Estofolete |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 03/10634-5 - A influencia da desnervacao mioenterica sobre a composicao estromal em tecido normal e em lesoes gastricas. |
| Beneficiário: | Carla Botelho Machado |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |