Pectinase production by a Brazilian thermophilic fungus Thermomucor indicae-seudaticae N31 in solid-state and submerged fermentation

Autor(es)

Martin, N. ^[1] ; Guez, M. A. U. ^[1] ; Sette, L. D. ^[2] ; Da Silva, R. ^[1] ; Gomes, E. ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Lab Bioquim & Microbiol Aplicada IBILCE, Sao Jose Do Rio Preto, SP - Brazil

^[2] UNICAMP Univ Campinas, Div Recursos Microbianos CPQBA, Paulinia, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROBIOLOGY; v. 79, n. 3, p. 306-313, JUN 2010.
• Área do conhecimento: Ciências Biológicas - Microbiologia
• Citações Web of Science: 21
• Assunto(s): Fungos termófilos Fermentação Poligalacturonase Thermoascus Aspergillus niger

Resumo

Thermophilic organisms produce thermostable enzymes, which have a number of applications, justifying the interest in the isolation of new thermophilic strains and study of their enzymes. Thirty-four thermophilic and thermotolerant fungal strains were isolated from soil, organic compost, and an industrial waste pile based on their ability to grow at 45A degrees C and in a liquid medium containing pectin as the only carbon source. Among these fungi, 50% were identified at the genus level as Thermomyces, Aspergillus, Monascus, Chaetomium, Neosartoria, Scopulariopsis, and Thermomucor. All isolated strains produced pectinase during solid-state fermentation (SSF). The highest polygalacturonase (PG) activity was obtained in the culture medium of thermophilic strain N31 identified as Thermomucor indicae-seudaticae. Under SSF conditions on media containing a mixture of wheat bran and orange bagasse (1 : 1) at 70% of initial moisture, this fungus produced the maximum of 120 U/ml of exo-PG, while in submerged fermentation (SmF) it produced 13.6 U/ml. The crude PG from SmF was more thermostable than that from SSF and exhibited higher stability in acidic pH. (AU)

• Processo FAPESP: 10/12624-0 - Aplicação de métodos físico-químicos e enzimáticos na sacarificação do bagaço de cana: estudos de microrganismos, processos fermentativos relacionados e métodos de hidrólise
• Beneficiário: Eleni Gomes
• Linha de fomento: Auxílio à Pesquisa - Temático