Role of phospholipase C and protein kinase C in Aspergillus nidulans during growth on pectin or glucose: Effects on germination and duplication cycle

Autor(es)

Chellegatti, M. A. S. C. ^[1] ; Yuvamoto, P. D. ^[1] ; Said, S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Ciencias Farmaceut, Fac Ciencias Farmaceut Ribeirao Preto, BR-05508 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Folia Microbiologica; v. 55, n. 3, p. 228-232, MAY 2010.
• Citações Web of Science: 5

Resumo

The effects of PLC and Pkc inhibitors on Aspergillus nidulans depend on the carbon source. PLC inhibitors Spm and C48/80 delayed the first nuclear division in cultures growing on glucose, but stimulated it in media supplemented with pectin. Less intense were these effects on the mutant transformed with PLC-A gene rupture (AP27). Neomycin also delayed the germination in cultures growing on glucose or pectin; however, on glucose, the nuclear division was inhibited whereas in pectin it was stimulated. These effects were minor in AP27. The effects of Ro-31-8425 and BIM (both Pkc inhibitors) were also opposite for cultures growing on glucose or pectin. On glucose cultures of both strains BIM delayed germination and the first nuclear division, whereas on pectin both parameters were stimulated. Opposite effects were also detected when the cultures were growing on glucose or pectin in the presence of Ro-31-8425. (AU)

• Processo FAPESP: 02/11874-7 - Alteracoes no desenvolvimento celular e morfologia de aspergillus nidulans induzidas pela fonte de carbono: as vias de transducao de sinais.
• Beneficiário: Suraia Said
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 02/11875-3 - Efeito de inibidores da fosfolipase C (PLC) sobre a divisão nuclear e a septação em Aspergillus nidulans
• Beneficiário: Priscila Dorigao Yuvamoto
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica