Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

MuRF1 is a muscle fiber-type II associated factor and together with MuRF2 regulates type-II fiber trophicity and maintenance

Texto completo
Autor(es):
Moriscot, Anselmo S. [1] ; Baptista, Igor L. [2, 1] ; Bogomolovas, Julius [2] ; Witt, Christian [2] ; Hirner, Stephanie [2] ; Granzier, Henk [3] ; Labeit, Siegfried [2]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Cell & Dev Biol, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil
[2] Univ Med Mannheim, Inst Integrat Pathophysiol, D-68167 Mannheim - Germany
[3] Univ Arizona, Dept Physiol, Tucson, AZ 85724 - USA
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Journal of Structural Biology; v. 170, n. 2, SI, p. 344-353, MAY 2010.
Citações Web of Science: 47
Resumo

MuRF1 is a member of the RBCC (RING, B-box, coiled-coil) superfamily that has been proposed to act as an atrogin during muscle wasting. Here, we show that MuRF1 is preferentially induced in type-II muscle fibers after denervation. Fourteen days after denervation, MuRF1 protein was further elevated but remained preferentially expressed in type-II muscle fibers. Consistent with a fiber-type dependent function of MuRF1, the tibialis anterior muscle (rich in type-II muscle fibers) was considerably more protected in MuRF1-KO mice from muscle wasting when compared to soleus muscle with mixed fiber-types. We also determined fiber-type distributions in MuRF1/MuRF2 double-deficient KO (dKO) mice, because MuRF2 is a close homolog of MuRF1. MuRF1/MuRF2 dKO mice showed a profound loss of type-II fibers in soleus muscle. As a potential mechanism we identified the interaction of MuRF1/MuRF2 with myozenin-1, a calcineurin/NFAT regulator and a factor required for maintenance of type-II muscle fibers. MuRF1/MuRF2 dKO mice had lost myozenin-1 expression in tibialis anterior muscle, implicating MuRF1/MuRF2 as regulators of the calcineurin/NFAT pathway. In summary, our data suggest that expression of MuRF1 is required for remodeling of type-II fibers under pathophysiological stress states, whereas MuRF1 and MuRF2 together are required for maintenance of type-II fibers, possibly via the regulation of myozenin-1. (C) 2010 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 07/57613-3 - Efeitos celulares e moleculares da sobrecarga de leucina na atrofia muscular induzida pelo desuso
Beneficiário:Igor Luchini Baptista
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 06/61523-7 - Aspectos celulares e moleculares da plasticidade muscular
Beneficiário:Anselmo Sigari Moriscot
Linha de fomento: Auxílio à Pesquisa - Temático