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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Acquisition of Serum Antibodies Reactive With Enterohemorrhagic Escherichia coli Virulence-Associated Factors by Healthy Brazilian Children and Adults

Texto completo
Autor(es):
Palmeira, Patricia [1] ; Carbonare, Solange B. [2] ; Guth, Beatriz E. C. [3] ; Carbonare, Cristiane B. [2] ; Pontes, Gerlandia N. [1] ; Tino-De-Franco, Milene [2] ; Zapata-Quintanilla, Lucy B. [2] ; Carneiro-Sampaio, Magda [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Med, Dept Pediat, BR-05403000 Sao Paulo - Brazil
[2] Butantan Inst, Immunogenet Lab, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: PEDIATRIC INFECTIOUS DISEASE JOURNAL; v. 28, n. 12, p. 1089-1094, DEC 2009.
Citações Web of Science: 6
Resumo

Background: Patients with hemorrhagic colitis or hemolytic uremic syndrome due to enterohemorrhagic Escherichia coli (EHEC) develop serum IgM and IgG response to lipopolysaccharide (LPS) and to virulence factors such as intimin. The small numbers of cases of diarrhea associated with EHEC strains in Brazil suggests a pre-existing immunity probably due to previous contact with diarrheagenic E. coli. Our aim was to evaluate the development of the serum antibody repertoire to EHEC virulence factors in Brazilian children and adults. Methods: Serum IgM and IgG antibodies were determined by enzyme-linked immunosorbent assay with LPS O111, LPS O26, and LPS O157 in 101 children between 2 months and 10 years of age and in 100 adult sera, by immunoblotting with protein membrane extracts and purified beta intimin; the ability of adult sera to neutralize Shiga toxin2 was also investigated. Results: Children older than 24 months had IgM concentrations reactive with the 3 LPS equivalent to those seen in the adult group, and significantly higher than the group of younger children (P < 0.05). Anti-O26 and anti-O157 LPS IgG concentrations were equivalent between the 2 groups of children and were significantly different from the adult group (P < 0.05). The anti-O111 LPS IgG levels in older children were intermediate between the younger group, and adults (P < 0.05). Immunoblotting revealed strong protein reactivity, including the conserved and variable regions of beta intimin and more than 50% of the adult samples neutralized Shiga toxin 2. Conclusions: Our results demonstrate an increasing anti-LPS and antiprotein antibody response with age, which could provide protection against EHEC infections. (AU)

Processo FAPESP: 02/03596-7 - A exposição a EPEC (E.coli enteropatogênicas) em áreas endêmicas pode proteger infecções por STEC (E.coli produtoras de toxina Shiga)? avaliação do desenvolvimento do repertório de anticorpos séricos..
Beneficiário:Patricia Palmeira Daenekas Jorge
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 03/13250-3 - Avaliação da atividade in vitro e in vivo de anticorpos humanos séricos e secretores anti-Escherichia coli enteropatogênicas e Escherichia coli produtoras de toxinas Shiga
Beneficiário:Solange Barros Carbonare
Linha de fomento: Auxílio à Pesquisa - Regular