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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS

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Autor(es):
Bicudo, Rogerio Campos [1] ; Bicudo, Tatiana Campos [2] ; Forato, Lucimara A. [3] ; Titato, Guilherme M. [1] ; Colnago, Luiz A. [3] ; Lancas, Fernando M. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Lab Cromatog, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Fed Rio Grande do Norte, Escola Ciencias & Tecnol, BR-59072970 Natal, RN - Brazil
[3] Embrapa Instrumentacao Agropecuaria, Sao Carlos, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF SEPARATION SCIENCE; v. 32, n. 21, SI, p. 3579-3584, NOV 2009.
Citações Web of Science: 1
Resumo

The nutritional value of maize seed is limited due to its high content of storage proteins (zeins), which are deficient in essential amino acids such as lysine and tryptophan. In a previous paper, we showed that protein bodies obtained from BR473 maize variety, developed by Embrapa (Brazilian Agricultural Research Corporation), were mainly constituted by Z27 and a smaller quantity of Z50 gamma-zeins. Besides zein proteins, other not identified protein band in the SDS/PAGE was also observed, which could indicate the presence of non-zein proteins additionally to gamma-zeins. In the present paper, we have demonstrated the presence of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS and database searching. This fact could be related to the excellent energetic value and higher protein quality of BR473 maize grains, since high lysine concentration in some maize varieties has been related to the presence of cytoskeleton proteins that are non-zeins. We have identified the following proteins: Brittle-1 protein (chloroplast precursor), Legumin-1, glyceroldehyde-3-phosphate dehydrogenase, and elongation factor 1-alpha. (AU)

Processo FAPESP: 02/08407-8 - Avaliação de sistemas de cromatografia líquida bidimensional acoplada a espectrometria de massas na análise do proteoma
Beneficiário:Rogerio de Campos Bicudo
Linha de fomento: Bolsas no Brasil - Doutorado Direto