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Ethanolic extract of Casearia sylvestris and its clerodane diterpen (caseargrewiin F) protect against DNA damage at low concentrations and cause DNA damage at high concentrations in mice's blood cells

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Autor(es):
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de Oliveira, Aline M. [1] ; dos Santos, Andre G. [2] ; dos Santos, Raquel A. [3] ; Csipak, Angelica R. [1] ; Olivato, Camila [1] ; da Silva, Isabel C. [1] ; de Freitas, Maura B. [1] ; Bassi, Carmen L. ; Cavalheiro, Alberto J. [4] ; Bolzani, Vanderlan S. [4] ; Silva, Dulce H. S. [4] ; Sakamoto-Hojo, Elza T. [3] ; Takahashi, Catarina S. [3] ; Soares, Christiane P. [1]
Número total de Autores: 14
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, Sch Pharmaceut Sci, Dept Clin Anal, Sao Paulo - Brazil
[2] Sao Paulo State Univ, Sch Pharmaceut Sci, Dept Principios Ativos Nat & Toxicol, Sao Paulo - Brazil
[3] Univ Sao Paulo, Dept Biol, Fac Philosophy Sci & Letters Ribeirao Preto, BR-05508 Sao Paulo - Brazil
[4] Sao Paulo State Univ, Inst Chem, Nucleo Bioensaios Biossintese & Ecofisiol Prod Na, Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: MUTAGENESIS; v. 24, n. 6, p. 501-506, NOV 2009.
Citações Web of Science: 12
Resumo

Casearia sylvestris is used in Brazil as a popular medicine to treat ulcer, inflammation and tumour. Caseargrewiin F is a clerodane diterpene isolated from the ethanolic leaf extract of C.sylvestris. The aim of the study was to assess the capacity of the ethanolic extract of C.sylvestris leaves and caseargrewiin F to protect DNA and verify if both the compounds cause some DNA damage, using the micronucleus (MN) test and comet assay in mice. Balb-C mice were treated with the extract {[}3.13, 6.25, 12.5, 25, 50 and 75 mg/kg body weight (b.w.)] and caseargrewiin F (0.16, 0.32, 0.63, 1.3, 2.5 and 3.8 mg/kg b.w.) for 14 days. On day 15, DNA damage was induced by intra-peritoneal (i.p.) injection of cyclophosphamide (CP) (i.p.) at 50 mg/kg b.w. after the MN test and comet assay were performed. A protective effect of ethanolic extract was observed in MN test (6.25 and 12.5 mg/kg b.w.) and the comet assay (3.13 and 6.25, 12.5 and 25 mg/kg b.w.). Caseargrewiin F showed protective effect at 0.63, 1.3 and 2.5 mg/kg b.w. only in comet assay. We also tested the ability of compounds of C.sylvestris to induce MN and to increase the comet assay tail moment. The experimental design was similar to the DNA protection assay except that in test groups we omitted the CP challenge. We observed increased damage at 50 and 75 mg/kg b.w. of ethanolic extract of C.sylvestris and caseargrewiin F at 3.18 mg/kg b.w. in both the MN test and comet assay. We conclude that ethanolic extract of C. sylvestris and caseargrewiin F can protect cells against DNA damage induced by CP at low concentrations, but at high concentrations these compounds also induce DNA damage. (AU)

Processo FAPESP: 03/02176-7 - Conservação e uso sustentável da diversidade do Cerrado e da Mata Atlântica: diversidade química e prospecção de medicamentos potenciais - fase II
Beneficiário:Vanderlan da Silva Bolzani
Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático
Processo FAPESP: 04/07932-7 - Busca de compostos naturais antitumorais, antioxidantes, antiinflamatórios, antidiabéticos, inibidores de acetilcolinesterase e mieloperoxidase no Cerrado e na Mata Atlântica
Beneficiário:Dulce Helena Siqueira Silva
Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático
Processo FAPESP: 05/58472-9 - Avaliação do efeito antimutagênico do extrato etanólico de Casearia sylvestris através do ensaio de micronúcleo em células sanguíneas de camundongo e células de inflorescência de Tradescantia pallida
Beneficiário:Aline Miranda de Oliveira
Linha de fomento: Bolsas no Brasil - Iniciação Científica