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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Immunohistochemical, tomographic and histological study on onlay bone graft remodeling. Part II: calvarial bone

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Pedrosa, Jr., Wagner Fernandes [1] ; Okamoto, Roberta [2] ; Pinto Faria, Paulo Esteves [2] ; Manfrin Arnez, Maya Fernanda [1] ; Xavier, Samuel Porfirio [1] ; Salata, Luiz Antonio [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Dent, Dept Oral & Maxillofacial Surg & Periodont, BR-14040904 Ribeirao Preto, SP - Brazil
[2] Univ State Sao Paulo Aracatuba, Fac Dent Aracatuba, Dept Oral & Maxillofacial Surg, Aracatuba, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Clinical Oral Implants Research; v. 20, n. 11, p. 1254-1264, NOV 2009.
Citações Web of Science: 37

Objectives Little information is available on the molecular events that occur during graft incorporation over time. The calvarial bone (Cb) grafts have been reported to produce greater responses compared with other donor regions in maxillofacial reconstructions, but the scientific evidences for this are still lacking. The objectives of this study are (1) to study the morphological pattern of Cb onlay bone grafts and compare them with the biological events through immunohistochemical responses and (2) to establish the effects of perforations in maintaining the volume and bone density of the receptor bed. Material and methods Sixty New Zealand White rabbits were submitted to Cb onlay bone grafts on the mandible. In 30 rabbits, the receptor bed was perforated (perforated group), while for the remaining animals the bed was kept intact (non-perforated group). Six animals from each group were sacrificed at 5, 7, 10, 20 and 60 days after surgery. Histological sections from the grafted area were prepared for immunohistochemical and histological analyses. Immuno-labeling was found for proteins Osteoprotegerin (OPG), receptor activator of nuclear factor-kappa beta ligand (RANKL), alkaline phosphatase (ALP), osteopontin (OPN), vascular endothelial growth factor (VEGF), tartrate-resistant acid phosphatase (TRAP), Type I collagen (COL I) and osteocalcin (OC). The tomography examination {[}computerized tomography (CT) scan] was conducted just after surgery and at the sacrifice. Results The histological findings revealed that the perforations contributed to higher bone deposition during the initial stages at the graft-receptor bed interface, accelerating the graft incorporation process. The results of the CT scan showed lower resorption for the perforated group (P < 0.05), and both groups showed high bone density rates at 60 days. This set of evidences is corroborated by the immunohistochemical outcomes indicating that proteins associated with revascularization and osteogenesis (VEGF, OPN, TRAP and ALP) were found in higher levels in the perforated group. Conclusions These findings indicate that the bone volume of calvarial grafts is better maintained when the receptor bed is perforated, probably resulting from more effective graft revascularization and greater bone deposition. The process of bone resorption peaked between 20 and 60 days post-operatively in both groups although significantly less in the perforated group. To cite this article:Pedrosa Jr WF, Okamoto R, Faria PEP, Arnez MFM, Xavier SP, Salata LA. Immunohistochemical, tomographic and histological study on onlay bone grafts remodeling. Part II: calvarial bone.Clin. Oral Impl. Res. 20, 2009; 1254-1264.doi: 10.1111/j.1600-0501.2009.01747.x. (AU)

Processo FAPESP: 06/01771-7 - Influência do processo de revascularização sobre a remodelação de enxertos ósseos onlay: estudo histométrico, imunohistoquímico e tomográfico em coelhos: Parte II - Calvária
Beneficiário:Luiz Antonio Salata
Linha de fomento: Auxílio à Pesquisa - Regular