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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Expression, purification, crystallization and preliminary crystallographic analysis of an endo-1,5-alpha-L-arabinanase from hyperthermophilic Thermotoga petrophila

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Autor(es):
Squina, Fabio Marcio [1] ; Prade, Rolf Alexander [2] ; Wang, Hongliang [2] ; Murakami, Mario Tyago [3]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Brazilian Assoc Synchrotron Light Technol, Bioethanol Sci & Technol Ctr, BR-13083970 Campinas, SP - Brazil
[2] Oklahoma State Univ, Dept Microbiol & Mol Genet, Stillwater, OK 74078 - USA
[3] Brazilian Assoc Synchrotron Light Technol, Ctr Struct Mol Biol, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS; v. 65, n. 9, p. 902-905, Aug. 2009.
Citações Web of Science: 8
Assunto(s):Enzimas   Bactérias   Thermotoga
Resumo

The endo-1,5-alpha-L-arabinanases belonging to glycoside hydrolase family 43 are of great industrial interest for use in food technology, organic synthesis and biofuel production owing to their ability to catalyze the hydrolysis of alpha-1,5-arabinofuranosidic bonds in arabinose-containing polysaccharides. In this work, Thermotoga petrophila endo-1,5-alpha-L-arabinanase, a GH43-family member, has been cloned, overexpressed, purified and crystallized. Single crystals were obtained from a solution containing 0.1 M MES buffer pH 6.5, 0.8 M ammonium sulfate, 0.1 M EDTA, 0.1 M l-proline and 5%(v/v) dioxane. X-ray diffraction data were collected to a resolution of 2.86 angstrom using synchrotron radiation and the diffraction pattern was indexed in the tetragonal space group P422, with unit-cell parameters a = b = 83.71, c = 408.25 angstrom. (AU)

Processo FAPESP: 08/58037-9 - Geração de biblioteca para conversão enzimática de biomassa a partir de metagenoma do solo
Beneficiário:Fábio Márcio Squina
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Apoio a Jovens Pesquisadores