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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Pulmonary Disease in Hamsters Infected with Leptospira interrogans: Histopathologic Findings and Cytokine mRNA Expressions

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Autor(es):
Marinho, Marcia [1] ; Oliveira-Junior, Itamar Souza [2] ; Rodrigues Monteiro, Cristina Maria ; Perri, Silvia Helena ; Salomao, Reinaldo [3]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, FOA, Microbiol Lab, Dept Anim Hlth & Prod, BR-16050680 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Inflammatory Mediators Lab, Geriatr & Gerontol Div, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Div Infect Dis, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: American Journal of Tropical Medicine and Hygiene; v. 80, n. 5, p. 832-836, MAY 2009.
Citações Web of Science: 19
Resumo

Our aim was to evaluate the pulmonary changes induced by Leptospira interrogans infection in hamsters, and the gene expression of endogenous mediators in lung fragments during 28 days of observation. The animals were euthanized on days 4, 7, 14, 21, and 28 post-inoculation. Histopathologic lung analysis showed hemorrhage, pneumonia, alveolar congestion, and infiltrated cellular areas, with increasing severity until day 21 post-inoculation. Tumor necrosis factor (TNF)-alpha mRNA expression enhanced in first days with peak on day 4 and slightly decreased in the final phase. The interleukin (IL)-10 remained relatively constant throughout the period, with the exceptions of days 4 and 14. The endothelial nitric-oxide synthesis (eNOS) showed an increased expression on day 4, followed by an augment on days 7 and 14, and remaining constant up to day 28 post-infection. Our results demonstrate that inoculation of L. interrogans sorovar Icterohaemorrhagiae induced pulmonary lesions, including pulmonary hemorrhage, Supporting that the lung is a target organ. (AU)

Processo FAPESP: 05/57085-1 - Determinação da produção de TNF-alfa, IL-1beta, iNOS, eNos e NO em macrófagos alveolares de hamsters inoculados com Leptospira interrogans
Beneficiário:Márcia Marinho
Modalidade de apoio: Auxílio à Pesquisa - Regular