Cohabitation with a B16F10 melanoma-bearer cage mate influences behavior and dendritic cell phenotype in mice

Autor(es)

Tomiyoshi, M. Y. ^[1] ; Sakai, M. ^[2] ; Baleeiro, R. B. ^[1] ; Stankevicius, D. ^[2] ; Massoco, C. O. ^[2] ; Palermo-Neto, J. ^[2] ; Barbuto, J. A. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Tumor Immunol, Dept Immunol, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Neuroimmunomodulat Res Grp, Dept Pathol, Sch Vet Med, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BRAIN BEHAVIOR AND IMMUNITY; v. 23, n. 4, p. 558-567, MAY 2009.
• Citações Web of Science: 13

Resumo

This study evaluated the effects of cohabitation with a B16F10 melanoma-bearer cage mate on behavior and immune functions in mice. Five different experiments were conducted. In each of them, the female mice were divided into two groups: control and experimental. One mouse of each control pair was kept undisturbed and called ``companion of health partner{''} (CHP). One mouse of each experimental pair was inoculated with B16FI0 cells and the other, the subject of this study, was called ``companion sick partner{''} (CSP). On Day 20 of cohabitation, behavior and immune parameters from CHP and CSP mice were analyzed. In comparison to the CHP, the CSP mice: (1) presented an increased general locomotion in the open field and a decreased exploration time and number of entries in the plus-maze open arms; (2) had an enhanced expression of the CD80 costimulatory molecule on Iab(+)CD11c(+) spleen cells, but no differences were found on lymph nodes cells; (3) presented an altered differentiation of bone marrow cells in the presence of GM-CSF, IL-4, and LPS in vitro, resulting in a lower percentage of Iab(+)CD80(+) cells; (4) had a deficit in the establishment of a Delayed Type of Hypersensitivity to ovalbumin, which was associated to an in vitro proliferation of an IL-10-producing lymphocyte subpopulation after ovalbumin stimulation. Corticosterone levels detected on Day 20 of cohabitation were similar in CHP and CSP mice. It is shown here that DCs phenotype in mice is affected by conditions associated with behavioral alterations indicative of an anxiety-like state induced by the cohabitation with a tumor-bearer conspecific. This phenomenon occurred probably through a nondependent corticosterone mechanism. (C) 2009 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 05/50574-7 - A influencia do "estresse" na diferenciacao e ativacao de celulas dendriticas em camundongos c57bi/6.
• Beneficiário: Marcio Yuiti Tomiyoshi
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 04/14128-0 - Neuroimunomodulação: efeitos do estresse e de citocinas nas relações bidirecionais entre os sistemas nervosos central e imune
• Beneficiário: João Palermo Neto
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 04/09956-0 - Modulação funcional de células dentríticas em diferentes situações fisiopatológicas
• Beneficiário: Jose Alexandre Marzagão Barbuto
• Modalidade de apoio: Auxílio à Pesquisa - Temático