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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Characterization of the Trypanosoma cruzi ortholog of the SBDS protein reveals an intrinsically disordered extended C-terminal region showing RNA-interacting activity

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Autor(es):
de Oliveir, Juliana Ferreira [1] ; Castilho, Beatriz A. [2] ; Sforca, Mauricio L. [1] ; Krieger, Marco Aurelio [3] ; Zeri, Ana Carolina [1] ; Guimaraes, Beatriz G. [1] ; Zanchin, Nilson I. T. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] LNLS, Brazilian Synchrotron Light Lab, Ctr Struct Mol Biol, BR-13083970 Campinas, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 Sao Paulo - Brazil
[3] IBMP, Curitiba, Parana - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Biochimie; v. 91, n. 4, p. 475-483, APR 2009.
Citações Web of Science: 2
Resumo

The human SBDS gene and its yeast ortholog SDOI encode essential proteins that are involved in ribosome biosynthesis. SDOI has been implicated in recycling of the ribosomal biogenesis factor Tif6p from pre-66S particles as well as in translation activation of 60S ribosomes. The SBDS protein is highly conserved, containing approximately 250 amino acid residues in animals, fungi and Archaea, while SBDS orthologs of plants and a group of protists contain an extended C-terminal region. In this work, we describe the characterization of the Trypanosoma cruzi SBDS ortholog (TcSBDS). TcSBDS co-fractionates with polysomes in sucrose density gradients, which is consistent with a role in ribosome biosynthesis. We show that TcSBDS contains a C-terminal extension of 200 amino acids that displays the features of intrinsically disordered proteins as determined by proteolytic, circular dichroism and NMR analyses. Interestingly, the C-terminal extension is responsible for TcSBDS-RNA interaction activity in electrophoretic mobility shift assays. This finding suggests that Trypanosomatidae and possibly also other organisms containing SBDS with extended C-terminal regions have evolved an additional function for SBDS in ribosome biogenesis. (C) 2008 Elsevier Masson SAS. All rights reserved. (AU)

Processo FAPESP: 06/02083-7 - Caracterização funcional de proteínas envolvidas no controle da expressão gênica
Beneficiário:Nilson Ivo Tonin Zanchin
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 00/10266-8 - A structural biology laboratory network for the study of the 3D structures of proteins
Beneficiário:Nilson Ivo Tonin Zanchin
Linha de fomento: Auxílio à Pesquisa - Programa GENOMA
Processo FAPESP: 98/14138-2 - Center for Structural Molecular Biotechnology
Beneficiário:Glaucius Oliva
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 02/12597-7 - Início da síntese protéica em eucariotos: mecanismos e regulação
Beneficiário:Beatriz Amaral de Castilho
Linha de fomento: Auxílio à Pesquisa - Temático