Ultrastructural Morphology and Morphometry of Phrenic Nerve in Rats

Autor(es)

Sassoli Fazan, Valeria Paula ^{[1, 2]} ; Rodrigues Filho, Omar Andrade ^[3] ; Rodrigues Jordao, Caroline Echavarria ^[2] ; Moore, Kenneth Charles ^[1]

Afiliação do(s) autor(es)

^[1] Univ Iowa, Cent Microscopy Res Facil, Iowa City, IA - USA

^[2] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Surg & Anat, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Univ Fed Triangulo Mineiro, Dept Biol Sci, Uberaba, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology; v. 292, n. 4, p. 513-517, APR 2009.
• Citações Web of Science: 6

Resumo

Despite numerous literature reports on the morphometry of the myelinated fibers of phrenic nerves in rats, a systematic study of the longitudinal and lateral symmetry of the unmyelinated fibers morphometry is not available. In this study, we have undertaken ultrastructural and morphometric studies of the phrenic nerve in adult rats, assessing two different levels (proximal and distal) from both right and left sides. Phrenic nerves of adult male Wistar rats were prepared for epoxy resin embedding and transmission electron microscopy. Morphometric analysis was performed with the aid of computer software, which took into consideration the unmyelinated fiber number, density, area, and diameter, as well as ratio between myelinated and unmyelinated fibers, and the percentage of the fascicular area occupied by the myelinated and unmyelinated fibers. Comparison of data from proximal and distal segments on the same side and from the same levels between sides was performed. Differences were considered significant when P < 0.05. The most important finding is that morphometric parameters of the phrenic nerve unmyelinated fibers in adult rats are both longitudinally and laterally symmetric. This study adds important morphometric information about the unmyelinated fibers of the phrenic nerves in adult rats for proximal and distal levels on both sides of the animal. Anat Rec, 292:513-517, 2009. (C) 2008 Wiley-Liss, Inc. (AU)

• Processo FAPESP: 06/06362-8 - Avaliação morfológica ultraestrutural das alterações do nervo renal de ratos diabéticos induzidos experimentalmente
• Beneficiário: Valéria Paula Sassoli Fazan
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 04/09139-2 - Estudos morfofuncionais e morfométricos do sistema nervoso central e periférico e do sistema cardiovascular, envolvidos na regulação neuro-humoral de funções neurovegetativas e comportamentais
• Beneficiário: Helio Cesar Salgado
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 07/08498-7 - Avaliação em nível de microscopia eletrônica de transmissão do nervo frênico de ratos diabéticos crônicos induzidos experimentalmente
• Beneficiário: Caroline Echavarria Rodrigues Jordão
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 06/03200-7 - Estudo morfológico e morfométrico do nervo sural de ratos normotensos, espontaneamente hipertensos (SHR) e espontaneamente hipertensos com diabete induzido experimentalmente
• Beneficiário: Valéria Paula Sassoli Fazan
• Modalidade de apoio: Auxílio à Pesquisa - Regular