The enzymatic nature of fungal bioluminescence

Autor(es)

Oliveira, Anderson G. ^[1] ; Stevani, Cassius V. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, BR-05599970 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. 8, n. 10, p. 1416-1421, 2009.
• Citações Web of Science: 31

Resumo

The uncertainty about the possible involvement of a luciferase in fungal bioluminescence has not only hindered the understanding of its biochemistry but also delayed the characterization of its constituents. The present report describes how in vitro light emission can be obtained enzymatically from the cold and hot extracts assay using different species of fungi, which also indicates a common mechanism for all these organisms. Kinetic data suggest a consecutive two-step enzymatic mechanism and corroborate the enzymatic proposal of Airth and Foerster. Finally, overlapping of light emission spectra from the fungal bioluminescence and the in vitro assay confirm that this reaction is the same one that occurs in live fungi. (AU)

• Processo FAPESP: 04/13856-1 - Estudo mecanístico da bioluminescência do fungo Gerronema viridilucens
• Beneficiário: Anderson Garbuglio de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 06/53628-3 - Estudo da bioluminescencia de fungos e suas aplicacoes em quimica ambiental.
• Beneficiário: Cassius Vinicius Stevani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 01/04753-6 - Bioluminescência e atividade farmacológica de cogumelos
• Beneficiário: Cassius Vinicius Stevani
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores