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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Interleukin-6 treatment enhances human monocyte permissiveness for Paracoccidioides brasiliensis growth by modulating cytokine production

Texto completo
Autor(es):
Siqueira, Keila Zaniboni [1] ; Victoriano De Campos Soares, Angela Maria [1] ; Dias-Melicio, Luciane Alarcao [1] ; Calvi, Sueli Aparecida [2] ; Serrao Peracoli, Maria Terezinha [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, Inst Biosci, Dept Microbiol & Immunol, Botucatu, SP - Brazil
[2] Sao Paulo State Univ, Sch Med, Dept Trop Dis & Diag Image, Botucatu, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Medical Mycology; v. 47, n. 3, p. 259-267, 2009.
Citações Web of Science: 8
Resumo

The effect of interleukin (IL)-6 on cytokine production was evaluated in human monocyte cultures infected with the virulent strain of Paracoccidioides brasiliensis (Pb18). Peripheral blood monocytes from healthy individuals were preincubated for 24 h with or without human recombinant IL-6, and then challenged with Pb18 for 4 h and 18 h. P. brasiliensis growth was assessed by viable fungi recovery from co-cultures after plating on BHI-agar. Moreover, tumor necrosis factor-alpha (TNF-), IL-6 and IL-10 production in supernatant cultures was determined by enzyme immunoassay (ELISA). Monocyte preincubation with IL-6, and challenged with Pb18 for 4 h, led to significantly higher fungi recovery compared to non-treated co-cultures. The pretreatment of monocytes with IL-6 induced an inhibitory effect on TNF- and IL-10 production during 18 h fungal infection. Otherwise, an autocrine stimulatory effect on IL-6 production was detected at 4 h and 18 h as represented by an elevation in IL-6 levels. The reduction in TNF- levels and stimulation of IL-6 production induced by previous IL-6 treatment might be responsible for a significant increase in fungal growth in human monocytes. The results suggest that IL-6, by exerting a modulatory effect on cytokines production, makes monocyte more permissive for fungal growth. (AU)

Processo FAPESP: 01/12418-2 - Efeito da interleucina-6 sobre a atividade fungicida de monócitos humanos, infectados in vitro com cepas de Paracoccidioides brasiliensis de alta e baixa virulência
Beneficiário:Keila Zaniboni Siqueira
Linha de fomento: Bolsas no Brasil - Mestrado