Silencing of mitochondrial alternative oxidase gene of Aspergillus fumigatus enhances reactive oxygen species production and killing of the fungus by macrophages

Autor(es)

Magnani, Taisa ^[1] ; Soriani, Frederico Marianetti ^[1] ; Martins, Vicente de Paulo ^[1] ; de Freitas Policarpo, Anna Carolina ^[1] ; Sorgi, Carlos Arterio ^[1] ; Faccioli, Lucia Helena ^[1] ; Curti, Carlos ^[2] ; Uyemura, Sergio Akira ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Quim & Fis, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Bioenergetics and Biomembranes; v. 40, n. 6, p. 631-636, DEC 2008.
• Citações Web of Science: 29

Resumo

We previously demonstrated that conidia from Aspergillus fumigatus incubated with menadione and paraquat increases activity and expression of cyanide-insensitive alternative oxidase (AOX). Here, we employed the RNA silencing technique in A. fumigatus using the vector pALB1/aoxAf in order to down-regulate the aox gene. Positive transformants for aox gene silencing of A. fumigatus were more susceptible both to an imposed in vitro oxidative stress condition and to macrophages killing, suggesting that AOX is required for the A. fumigatus pathogenicity, mainly for the survival of the fungus conidia during host infection and resistance to reactive oxygen species generated by macrophages. (AU)

• Processo FAPESP: 03/09781-3 - Clonagem, expressão e caracterização do gene da oxidase alternativa mitocondrial de Aspergillus fumigatus
• Beneficiário: Taisa Magnani Dinamarco
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 06/04909-0 - Vias alternativas mitocondriais: estudos de NADH desidrogenase alternativa e oxidase alternativa em fungos
• Beneficiário: Sergio Akira Uyemura
• Modalidade de apoio: Auxílio à Pesquisa - Regular