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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Renal contribution to thermolability in rats: role of renal nerves

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Autor(es):
Lutaif, Nelson A. [1] ; Rocha, Eduardo M. [1] ; Veloso, Leda A. [1] ; Bento, Leda M. [1] ; Gontijo, Jose A. R. [2]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Lab Metab Hidro Salino Nucl Med & Cirurgia Expt, Dept Clin Med, Disciplina Med Interna, BR-13083592 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Fac Ciencias Med, Dept Clin Med, BR-13083592 Campinas, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Nephrology Dialysis Transplantation; v. 23, n. 12, p. 3798-3805, DEC 2008.
Citações Web of Science: 4
Resumo

Background. Body temperature is closely regulated via the integration of a number of mechanisms, the study of which has been greatly assisted by the exploitation of comparative physiology. Previous studies have demonstrated that chronic renal failure patients have significantly lower body temperatures than healthy subjects when artifacts from circadian changes were taken into consideration. We hypothesize that the blunting of renal sensory neurons after kidney partial ablation may contribute to the lack of suppression of sympathetic efferent outflow towards BAT, modifying the glucose metabolism signaling pathway, UCP 1 expression and liver mitocondrial respiratory chain activity. Methods. To evaluate the influence of renal mass reduction, renal denervation and chronic deafferentation by capsaicin on thermoregulation, glucose metabolism, UCP1 expression and liver mitocondrial respiration, was used respectively, the blocking of heat dissipation by thermoneutral body water immersion, the oxygen consumption by Clark-type electrode, and western blot method. Results. The study confirmed that, following 5/6 nephrectomy, the basal core temperature of rats was significantly lower than that of control animals when maintained in a thermoneutral body water immersion recipient, as compared to controls. Additionally, we demonstrated that exposure of bilateral renal denervated or of renal chronic capsaicin-treated rats to a similar experimental protocol results in a fast and high rise in rectal temperature response, and this is associated with a significant increase in the basal serine phosphorylation and protein levels of Akt and protein levels of UCP1. This was observed despite unchanged liver mitochondria respiratory control and ADP/O ratios in 5/6 Nx, as well as DNx, when compared to control mitochondria. Conclusions. Speculatively, it may be suggested that one of the renal sensory nerve signal defects associated with decreased kidney energy generation, induced by kidney ablation, may result in an inability to control the body temperature. (AU)

Processo FAPESP: 06/52431-1 - Nefrogenese em um modelo genetico de hipertensao arterial primaria: implicacoes no desenvolvimento hipertensivo de ratos espontaneamente hipertensos (shr).
Beneficiário:Jose Antonio Rocha Gontijo
Linha de fomento: Auxílio à Pesquisa - Regular