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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The macrophage-derived neutrophil chemotactic factor, MNCF: A lectin with TNF-alpha-like activities on neutrophils

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Autor(es):
Toledo, Karina Alves [1] ; Pereira, Fernando Lourenco [1] ; Mambole, Agnes [2] ; Lesavre, Philippe [2] ; Roque-Barreira, Maria Cristina [1] ; Halbwachs-Mecarelli, Lise [2]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, FMRP, Dept Biol Celular Mol, BR-14049900 Ribeirao Preto - Brazil
[2] Univ Paris 05, INSERM, U845, F-75015 Paris - France
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Biochemical and Biophysical Research Communications; v. 376, n. 4, p. 764-769, NOV 28 2008.
Citações Web of Science: 1
Resumo

The macro phage-derived neutrophil chemotactic factor (MNCF) is an alpha-galactoside-binding lectin, known to induce dexamethasone-insensitive neutrophil recruitment. We further characterized MNCF effects on neutrophils and showed that it shares with TNF-alpha the ability to delay apoptosis and to trigger degranulation. MNCF and TNF-alpha effects show similar kinetics and involve Src kinases and MAPKinases dependent pathways. They were, however, clearly distinguished, since the soluble TNF-receptor etanercept prevented TNF but not MNCF effects, while melibiose disaccharide inhibited MNCF but not TNF effects. Absorption of MNCF on detoxi-gel did not alter its properties, precluding an LPS contamination effect. By contrast, galectin-3 required LPS to activate neutrophils. Specific antibodies allowed to further demonstrate that MNCF and galectin-3 are two distinct molecules. Finally, MNCF- and IL-8-induced neutrophil activation differed by their kinetic and sensitivity to pertussis toxin. In conclusion, MNCF is a distinct neutrophil agonist, with pro-inflammatory activities involving its carbohydrate recognition domain. (C) 2008 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 06/60642-2 - Efeitos biológicos e aplicações farmacêuticas de lectinas
Beneficiário:Maria Cristina Roque Antunes Barreira
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 03/12252-2 - Mediadores e moléculas de adesão envolvidas na atividade inflamatória da lectina MNCF
Beneficiário:Karina Alves de Toledo
Linha de fomento: Bolsas no Brasil - Doutorado