Composition of supra- and subgingival biofilm of subjects with healthy and diseased implants

Autor(es)

Shibli, Jamil A. ^[1] ; Melo, Leandro ^[1] ; Ferrari, Daniel S. ^[1] ; Figueiredo, Luciene C. ^[1] ; Faveri, Marcelo ^[1] ; Feres, Magda ^[1]

Afiliação do(s) autor(es)

^[1] Univ Guarulhos, Dent Res Div, Dept Periodontol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Clinical Oral Implants Research; v. 19, n. 10, p. 975-982, OCT 2008.
• Citações Web of Science: 168

Resumo

Objectives: The purpose of this study was to compare the microbial composition of supra- and subgingival biofilm in subjects with and without peri-implantitis. Material and methods: Forty-four subjects (mean age 48.9 +/- 13.51 years) with at least one implant restored and functional for at least 2 years were assigned to two groups: a peri-implantitis group (n=22), consisting of subjects presenting peri-implant sites with radiographic defects > 3 mm, bleeding on probing and/or suppuration; and a control group (n=22), consisting of subjects with healthy implants. The clinical parameters evaluated were plaque index, gingival bleeding, bleeding on probing, suppuration, probing depth and clinical attachment level. Supra- and subgingival biofilm samples were taken from the deepest sites of each implant and analyzed for the presence of 36 microorganisms by checkerboard DNA-DNA hybridization. Results: Higher mean counts of Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia were observed in the peri-implantitis group, both supra- and subgingivally (P < 0.05). The proportions of the pathogens from the red complex were elevated, while host-compatible beneficial microbial complexes were reduced in diseased compared with healthy implants. The microbiological profiles of supra- and subgingival environments did not differ substantially within each group. Conclusion: Marked differences were observed in the composition of supra- and subgingival biofilm between healthy and diseased implants. The microbiota associated with peri-implantitis was comprised of more periodontal pathogenic bacterial species, including the supragingival biofilm. (AU)

• Processo FAPESP: 03/05023-7 - Avaliação dos parâmentros clínicos, microbiológicos e imunológicos de implantes osseointegrados
• Beneficiário: Jamil Awad Shibli
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/01939-2 - Metronidazol associado à amoxicilina no tratamento das peri-implantites
• Beneficiário: Jamil Awad Shibli
• Linha de fomento: Auxílio à Pesquisa - Regular