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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Plasmodium vivax apical membrane antigen-1: comparative recognition of different domains by antibodies induced during natural human infection

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Autor(es):
Mufalo, Bruno C. [1] ; Gentil, Fernanda [1] ; Bargieri, Daniel Y. [2] ; Costa, Fabio T. M. [3] ; Rodrigues, Mauricio M. [2] ; Soares, Irene S. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, BR-05508900 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Escola Paulista Med, CINTERGEN, BR-04044010 Sao Paulo - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Parasitol, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Microbes and Infection; v. 10, n. 12-13, p. 1266-1273, OCT 2008.
Citações Web of Science: 27
Resumo

The Apical Membrane Antigen-1 (AMA-1) of Plasmodium sp. has been suggested as a vaccine candidate against malaria. This protein seems to be involved in merozoite invasion and its extra-cellular portion contains three distinct domains: DI, DII, and DIII. Previously, we described that Plasmodium vivax AMA-1 (PvAMA-1) ectodomain is highly immunogenic in natural human infections. Here, we expressed each domain, separately or in combination (DI-II or DII-III), as bacterial recombinant proteins to map immunodominant epitopes within the PvAMA-1 ectodomain. IgG recognition was assessed by ELISA using sera of P. vivax-infected individuals collected from endemic regions of Brazil or antibodies raised in immunized mice. The frequencies of responders to recombinant proteins containing the DII were higher than the others and similar to the ones observed against the PvAMA-1 ectodomain. Moreover, ELISA inhibition assays using the PvAMA-1 ectodomain as substrate revealed the presence of many common epitopes within DI-II that are recognized by human immune antibodies. Finally, immunization of mice with the PvAMA-1 ectodomain induced high levels of antibodies predominantly to DI-II. Together, our results indicate that DII is particularly immunogenic during natural human infections, thus indicating that this region could be used as part of an experimental sub-unit vaccine to prevent vivax malaria. (C) 2008 Elsevier Masson SAS. All rights reserved. (AU)

Processo FAPESP: 04/00768-7 - Estudo do reconhecimento imune e diversidade antigênica do antígeno 1 de menbrana apical (AMA-1) de Plasmodium vivax em áreas endêmicas de Malária do Brasil
Beneficiário:Irene da Silva Soares
Linha de fomento: Auxílio à Pesquisa - Regular