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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Expression of a hantavirus N protein and its efficacy as antigen in immune assays

Texto completo
Autor(es):
Figueiredo, L. T. M. [1] ; Moreli, M. L. [1] ; Borges, A. A. [1] ; Figueiredo, G. G. [1] ; Souza, R. L. M. [1] ; Aquino, V. H. [2]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] USP, FMRP, Ctr Pesquisa Virol, BR-14040900 Ribeirao Preto, SP - Brazil
[2] USP, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut, BR-14040900 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Brazilian Journal of Medical and Biological Research; v. 41, n. 7, p. 596-599, JUL 2008.
Citações Web of Science: 28
Resumo

Hantavirus cardiopulmonary syndrome (HCPS) has been recognized as an important public heath problem. Five hantaviruses associated with HCPS are currently known in Brazil: Juquitiba, Araraquara, Laguna Negra-like, Castelo dos Sonhos, and Anajatuba viruses. The laboratory diagnosis of HCPS is routinely carried out by the detection of anti-hantavirus IgM and/or IgG antibodies. The present study describes the expression of the N protein of a hantavirus detected in the blood sample of an HCPS patient. The entire S segment of the virus was amplified and found to be 1858 nucleotides long, with an open reading frame of 1287 nucleotides that encodes a protein of 429 amino acids. The nucleotide sequence described here showed a high identity with the N protein gene of Araraquara virus. The entire N protein was expressed using the vector pET200D and the Escherichia coli BL21 strain. The expression of the recombinant protein was confirmed by the detection of a 52-kDa protein by Western blot using a pool of human sera obtained from HCPS patients, and by specific IgG detection in five serum samples of HCPS patients tested by ELISA. These results suggest that the recombinant N protein could be used as an antigen for the serological screening of hantavirus infection. (AU)

Processo FAPESP: 02/08459-8 - Rede de Diversidade Genética de Vírus (VIRGEN), selecionado para Laboratório L3: projeto: estudos com Hantavirus e vírus da dengue
Beneficiário:Luiz Tadeu Moraes Figueiredo
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 03/03682-3 - Estudos sobre arboviroses e viroses associadas a roedores
Beneficiário:Luiz Tadeu Moraes Figueiredo
Linha de fomento: Auxílio à Pesquisa - Temático