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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Aggregatibacter actinomycetemcomitans arcB INFLUENCES HYDROPHOBIC PROPERTIES, BIOFILM FORMATION AND ADHESION TO HYDROXYAPATITE

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Autor(es):
Longo, P. L. [1] ; Ota-Tsuzuki, C. [1] ; Nunes, A. C. R. [1] ; Fernandes, B. L. [1] ; Mintz, K. [2] ; Fives-Taylor, P. [2] ; Mayer, M. P. A. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo - Brazil
[2] Univ Vermont, Dept Mol Genet, Burlington, VT - USA
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Brazilian Journal of Microbiology; v. 40, n. 3, p. 550-562, SEP 2009.
Citações Web of Science: 2
Resumo

The regulation of gene expression in the oral pathogen Aggregatibacter actinomycetemcomitans is still not fully elucidated. ArcAB is a two-component system which allows facultative anaerobic bacteria to sense various respiratory growth conditions and adapt their gene expression accordingly. This study investigated in A. actinomycetemcomitans the role of ArcB on the regulation of biofilm formation, adhesion to saliva coated hydroxyapatite (SHA) and the hydrophobic properties of the cell. These phenotypic traits were determined for an A. actinomycetemcomitans arcB deficient type and a wild type strain. Differences in hydrophobic properties were shown at early and late exponential growth phases under microaerobic incubation and at late exponential phase under anaerobiosis. The arcB mutant formed less biofilm than the wild type strain when grown under anaerobic incubation, but displayed higher biofilm formation activity under microaerobic conditions. The adherence to SHA was significantly lower in the mutant when compared with the wild type strain. These results suggest that the transmembrane sensor kinase ArcB, in A. actinomycetemcomitans, senses redox growth conditions and regulates the expression of surface components of the bacterial cell related to biofilm formation and adhesion to saliva coated surfaces. (AU)

Processo FAPESP: 03/08598-0 - Fatores de virulência de A. actinomycetemcomitans: participação na doença, regulação da expressão, diversidade e resposta imune
Beneficiário:Marcia Pinto Alves Mayer
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 03/01192-9 - Análise da expressão gênica mediada pelo operon ARC em Actinobacillus actinomycetemcomitans
Beneficiário:Priscila Larcher Longo
Linha de fomento: Bolsas no Brasil - Doutorado