Development and characterization of 15 microsatellite loci for Cariniana estrellensis and transferability to Cariniana legalis, two endangered tropical tree species

Autor(es)

Guidugli, Marcela Corbo ^[1] ; de Campos, Tatiana ^[2] ; Barbosa de Sousa, Adna Cristina ^[2] ; Feres, Juliana Massimino ^[1] ; Sebbenn, Alexandre Magno ^[3] ; Mestriner, Moacyr Antonio ^[1] ; Betioli Contel, Eucleia Primo ^[1] ; Alzate-Marin, Ana Lilia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Lab Genet Vegetal, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Dept Genet & Evolucao, BR-13083970 Campinas, SP - Brazil

^[3] Inst Florestal Sao Paulo, BR-01059970 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CONSERVATION GENETICS; v. 10, n. 4, p. 1001-1004, AUG 2009.
• Citações Web of Science: 11

Resumo

From a genomic library enriched for GA/CA repeats, 15 highly polymorphic microsatellite markers were developed for Cariniana estrellensis, a tropical forest tree. The microsatellite loci were screened in 49 mature trees found between Pardo river and Mogi-Gua double dagger u river basins, in the state of So Paulo, Brazil. A total of 140 alleles were detected with an average of 9.33 alleles per locus. The expected heterozygosity ranged from 0.37 to 0.88. These loci showed a high probability of paternity exclusion. Additionally, 12 loci were effectively transferred to Cariniana legalis. High levels of polymorphism make the present SSR markers useful for population genetic studies. (AU)

• Processo FAPESP: 03/04199-4 - Uso de marcadores SSR na análise genética de árvores matrizes vs. populações derivadas que formam parte do Banco de Germoplasma da Floresta da USP-RP
• Beneficiário: Ana Lilia Alzate Marin
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
• Processo FAPESP: 07/04787-4 - Fluxo de pólen e isolamento genético de Cariniana estrellensis (Raddi) Kuntze (Lecythidaceae) em fragmento localizado no Interior de São Paulo
• Beneficiário: Marcela Corbo Guidugli
• Linha de fomento: Bolsas no Brasil - Doutorado