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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Gene expression profile of the plant pathogen Xylella fastidiosa during biofilm formation in vitro

Autor(es):
Souza, Alessandra A. de ; Takita, Marco A. ; Coletta-Filho, Helvécio D. ; Caldana, Camila ; Yanai, Giane M. ; Muto, Nair H. ; Oliveira, Regina C. de ; Nunes, Luiz Roberto [8] ; Machado, Marcos A.
Número total de Autores: 9
Tipo de documento: Artigo Científico
Fonte: FEMS Microbiology Letters; v. 237, n. 2, p. 341-353, Aug. 2004.
Área do conhecimento: Ciências Biológicas - Bioquímica
Assunto(s):Análise de sequência de DNA   Biofilmes   Xilema   Bactérias   Xylella fastidiosa
Resumo

A biofilm is a community of microorganisms attached to a solid surface. Cells within biofilms differ from planktonic cells, showing higher resistance to biocides, detergent, antibiotic treatments and host defense responses. Even though there are a number of gene expression studies in bacterial biofilm formation, limited information is available concerning plant pathogen. It was previously demonstrated that the plant pathogen Xylella fastidiosa could grow as a biofilm, a possibly important factor for its pathogenicity. In this study we utilized analysis of microarrays to specifically identify genes expressed in X. fastidiosa cells growing in a biofilm, when compared to planktonic cells. About half of the differentially expressed genes encode hypothetical proteins, reflecting the large number of ORFs with unknown functions in bacterial genomes. However, under the biofilm condition we observed an increase in the expression of some housekeeping genes responsible for metabolic functions. We also found a large number of genes from the pXF51 plasmid being differentially expressed. Some of the overexpressed genes in the biofilm condition encode proteins involved in attachment to surfaces. Other genes possibly confer advantages to the bacterium in the environment that it colonizes. This study demonstrates that the gene expression in the biofilm growth condition of the plant pathogen X. fastidiosa is quite similar to other characterized systems. (AU)

Processo FAPESP: 99/04266-6 - Expressed genes of Xylella fastidiosa at slow and fast growing conditions
Beneficiário:Marcos Antonio Machado
Linha de fomento: Auxílio à Pesquisa - Programa GENOMA
Processo FAPESP: 98/16257-9 - Identification and functional analysis of oxidative stress response genes in Xylella fastidiosa
Beneficiário:Regina Lucia Batista da Costa de Oliveira
Linha de fomento: Auxílio à Pesquisa - Programa GENOMA