Inhibition of avian metapneumovirus (AMPV) replication by RNA interference targeting nucleoprotein gene (N) in cultured cells

Autor(es)

Ferreira‚ H.L. ; Spilki‚ F.R. ; Almeida‚ R.S. ; Santos‚ M.M.A.B. ; Arns‚ C.W.

• Tipo de documento: Artigo Científico
• Fonte: Antiviral Research; v. 74, n. 1, p. 77-81, 2007.

Resumo

Avian metapneumovirus (AMPV) is the primary causative agent of severe rhinotracheitis in turkeys. It is associated with swollen head syndrome in chickens and is the source of significant economic losses to animal food production. In this study, we designed specific short interfering RNA (siRNA) targeting the AMPV nucleoprotein (N) and fusion (F) genes. Three days post-virus infection, virus titration, real time RT-PCR, and RT-PCR assays were performed to verify the effect of siRNA in AMPV replication. A marked decrease in virus titers from transfected CER cells treated with siRNA/N was observed. Also, the production of N, F, and G mRNAs in AMPV was decreased. Results indicate that N-specific siRNA can inhibit virus replication. In future studies, a combination of siRNAs targeting the RNA polymerase complex may be used as a tool to study AMPV replication and/or antiviral therapy. (c) 2006 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 03/14012-9 - O efeito da aplicação do RNAi na infecção pelo pneumovírus aviário
• Beneficiário: Helena Lage Ferreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto