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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

High-risk human papillomavirus in oral squamous cell carcinoma of young patients

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Autor(es):
Kaminagakura, Estela [1] ; Villa, Luisa Lina [2] ; Andreoli, Maria Antonieta [2] ; Sobrinho, Joao Simao [2] ; Vartanian, Jose Guilherme [1] ; Soares, Fernando Augusto [3] ; Nishimoto, Ines Nobuko [1] ; Rocha, Rafael [3] ; Kowalski, Luiz Paulo [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] AC Camargo Hosp, Dept Head & Neck Surg & Otorhinolaryngol, BR-01509900 Sao Paulo - Brazil
[2] Ludwig Inst Canc Res, Sao Paulo - Brazil
[3] AC Camargo Hosp, Dept Anat Pathol, BR-01509900 Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: International Journal of Cancer; v. 130, n. 8, p. 1726-1732, APR 15 2012.
Citações Web of Science: 34
Resumo

The aim of this study was to investigate a possible relation between oral squamous cell carcinoma (SCC), the presence of high-risk human papillomavirus (HR-HPV) DNA and p16 expression in young patients. Paraffin-embedded tumor blocks from 47 oral SCC of young (<= 40-year old) patients were evaluated. The presence of HPV DNA in tumor specimens was analyzed by polymerase chain reaction (PCR) using GP5+/GP6+ generic primers (L1 region) followed by dot blot hybridization for HPV typing. When necessary, the HPV16 positivity was confirmed by PCR HPV16 E7-specific primers. Cases involving young patients were compared with 67 oral SCC from patients >= 50-year old (controls). Demographic and clinical data were collected to analyze patient outcomes. p16(ink4) expression was evaluated by immunostaining of tissue microarrays. HPV16 was detected in 22 (19.2%) cases; 15 (68.2%) young and 7 (31.8%) control patients, a statistically significant difference (p = 0.01). In 1 (1.7%) young group specimen, HPV DNA 16 and 18 was detected. p16 expression was observed in 11 (25.6%) cases from the young group and in 11 (19.6%) controls (p = 0.48). Association between HPV and p16 was verified, and it was statistically significant (p = 0.002). The higher prevalence of high-risk HPV types, especially HPV16, may be a contributing factor to oral carcinogenesis in younger individuals. (AU)

Processo FAPESP: 07/56117-2 - Aspectos clinicopatológicos e moleculares do carcinoma epidermóide bucal em pacientes com idade menor ou igual a 40 anos
Beneficiário:Luiz Paulo Kowalski
Linha de fomento: Auxílio à Pesquisa - Regular