Effects of periodontal therapy on GCF cytokines in generalized aggressive periodontitis subjects

Autor(es)

de Lima Oliveira, Ana Paula ^[1] ; de Faveri, Marcelo ^[2] ; Gursky, Lauren Christine ^[3] ; Mestnik, Maria Josefa ^[2] ; Feres, Magda ^[2] ; Haffajee, Anne D. ; Socransky, Sigmund S. ; Teles, Ricardo Palmier ^{[4, 5]}

Afiliação do(s) autor(es)

^[1] Univ Fed Minas Gerais, Sch Dent, Dept Periodontol, Belo Horizonte, MG - Brazil

^[2] Univ Guarulhos, Dept Periodontol, Dent Res Div, Guarulhos, SP - Brazil

^[3] Pontificia Univ Catolica Parana, Sch Dent, Curitiba, PR - Brazil

^[4] Forsyth Inst, Dept Periodontol, Cambridge, MA 02142 - USA

^[5] Harvard Univ, Sch Dent Med, Dept Oral Med Infect & Immun, Boston, MA 02115 - USA

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CLINICAL PERIODONTOLOGY; v. 39, n. 3, p. 295-302, MAR 2012.
• Citações Web of Science: 50

Resumo

Aim: To examine changes in levels of gingival crevicular fluid (GCF) cytokines, after periodontal therapy of generalized aggressive periodontitis (GAgP). Materials and Methods: Twenty-five periodontally healthy and 24 GAgP subjects had periodontal clinical parameters measured and gingival crevicular fluid (GCF) samples collected from up to 14 sites/subject. GCF samples were analysed using multiplex bead immunoassay for: GM-CSF, IFN-gamma, IL-10, IL-1 beta, IL-2, IL-6 and TNF-alpha. Aggressive periodontitis subjects were randomly assigned to either scaling and root planing (SRP) alone or SRP plus systemic amoxicillin (500 mg) and metronidazole (400 mg) 3 times a day for 14 days. Clinical parameters and GCF cytokines were re-measured 6 months after treatment. Differences over time were analysed using the Wilcoxon test and between groups using the Mann-Whitney test. Results: Significant reductions in GCF GM-CSF, IL-1 beta and the ratio IL-1 beta/IL-10 and increases in GCF IL-6 were detected after therapy. The mean change in GCF cytokines did not differ significantly between groups. Conclusions: Periodontal therapy improved GCF cytokine profiles by lowering IL-1 beta and increasing IL-10 levels. The reduction in GCF GM-CSF after therapy implicates this cytokine in the pathogenesis of GAgP. There was no difference between therapies in changes of GCF cytokines. (AU)

• Processo FAPESP: 07/56413-0 - Efeitos clínicos e microbiológicos da associação sistêmica de amoxicilina e metronidazol à raspagem e alisamento radicular em indivíduos com doença periodontal agressiva generalizada
• Beneficiário: Marcelo de Faveri
• Modalidade de apoio: Auxílio à Pesquisa - Regular