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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Immune Outcomes of Paradoxical Sleep Deprivation on Cellular Distribution in Naive and Lipopolysaccharide-Stimulated Mice

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Autor(es):
Zager, Adriano [1] ; Ruiz, Francieli Silva [1] ; Tufik, Sergio [1] ; Andersen, Monica Levy [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo UNIFESP EPM, Dept Psychobiol, Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: NEUROIMMUNOMODULATION; v. 19, n. 2, p. 79-87, 2012.
Citações Web of Science: 16
Resumo

Background/Aims: Several lines of evidence indicate that sleep loss imposes significant consequences on the host defense system, including changes in cell number, activity and distribution. However, it is not clear whether cellular alterations after sleep deprivation are caused by redistribution to immune organs or by death of these cells or how the response to a nonspecific immune activator would be affected. Therefore, the aim of this study was to assess the leukocyte distribution after paradoxical sleep deprivation (PSD) in saline-and lipopolysaccharide-treated mice. Methods: Adult inbred mice were paradoxical sleep deprived (72 h), whereas the controls were kept in their home cages. After PSD, both groups received an injection of either saline or lipopolysaccharide (LPS; 1 or 5 mu g/animal, intraperitoneally), 2 h prior to the collection of blood, spleen, lymph nodes and peritoneal wash. Isolated cells were then designated to differential leukocyte count (blood) and flow cytometry analysis of immune cell subsets (immune sites). Results: The data revealed that PSD caused a significant reduction of circulating lymphocytes and a general decrease in all cellular subsets of spleen, mainly T and B cells. However, no alteration in response of PSD was found on other immune sites, such as lymph nodes and peritoneum. Of note, immune cell distribution in response to in vivo LPS stimulation remained unchanged after PSD. Conclusions: Our study provided original evidence concerning the immune outcomes of PSD, indicating that cellular decrease caused by PSD is not restricted to circulation, but also to immune sites. Taken together, our results could help shed light on the physiological mechanisms of leukocyte trafficking. Copyright (C) 2012 S. Karger AG, Basel (AU)

Processo FAPESP: 98/14303-3 - Center for Sleep Studies
Beneficiário:Sergio Tufik
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 06/58275-1 - Efeitos da privação de sono na resposta ao lipopolissacarídeo bacteriano: aspectos comportamentais, neuroquímicos e imunológicos em camundongos
Beneficiário:Adriano Zager
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 07/55445-6 - Sono e imunidade: evidências em voluntários saudáveis e em modelo animal de transplante de pele
Beneficiário:Francieli Ruiz da Silva
Linha de fomento: Bolsas no Brasil - Doutorado Direto