Detection of etiological agents of malaria in howler monkeys from Atlantic Forests, rescued in regions of Sao Paulo city, Brazil

Autor(es)

Yamasaki, Tasciane ^{[1, 2]} ; Duarte, Ana M. R. C. ^[3] ; Curado, Izilda ^[4] ; Summa, Maria E. L. ^[5] ; Neves, Dafne V. D. A. ^[5] ; Wunderlich, Gerhard ^[6] ; Malafronte, Rosely S. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Med Trop Sao Paulo, Lab Protozool, BR-05403000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Dept Doencas Infecciosas & Parasitarias, BR-05403000 Sao Paulo - Brazil

^[3] Superintendencia Controle Endemias SUCEN, Lab Bioquim & Biol Mol, Sao Paulo - Brazil

^[4] Superintendencia Controle Endemias SUCEN, Lab Imunoepidemiol, Sao Paulo - Brazil

^[5] Prefeitura Municipio Sao Paulo DEPAVE PMSP, Secretaria Verde & Meio Ambiente, Dept Parques & Areas Verdes, Sao Paulo - Brazil

^[6] Univ Sao Paulo, ICB, Mol Biol Lab, BR-05403000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MEDICAL PRIMATOLOGY; v. 40, n. 6, p. 392-400, DEC 2011.
• Citações Web of Science: 25

Resumo

Background In some states of the Brazilian extra-Amazonian region, such as the Atlantic Forest area, autochthonous human cases of malaria were related to simian malarias and vice versa. Methods To verify the presence of Plasmodium, 50 blood samples of howler monkeys (Alouatta guariba clamitans) rescued from the Metropolitan Region of Sao Paulo city, where the Atlantic Forest is present, were analyzed. The samples were submitted to microscopy (thin and thick blood smears), enzyme-linked immunosorbent assays (ELISA), indirect immunofluorescent assay (IFA), and polymerase chain reaction (PCR). Results Only one smear showed forms reminiscent of Plasmodium vivax. In ELISA, the frequencies of antibodies against synthetic peptides corresponding to circumsporozoite protein of P. vivax VK210 `classic' (Pvc), P. vivax VK247, human P. vivax-like (Pvk and Pvl), P. malariae/P. brasilianum (Pm), and P. falciparum (Pf) were 24.0% (12/50) for Pvc, 8.0% (04/50) for Pvk, 6.0% (03/50) for Pvl, 24.0% (12/50) for Pm, and 28.0% (14/50) for Pf, while the frequency of antibodies against PvMSP119 recombinant proteins was 42.0% (21/50). No serum reacted against PfMSP1-19. In IFA, the seropositivity of antibodies against asexual forms of P. malariae was 31.3% (15/48). We utilized three PCR protocols to develop a molecular consensus (positive results in, at least, two protocols). The frequency of Plasmodium infections detected by PCR was 18.0% (09/50) for P. vivax, 4.0% (02/50) for P. malariae, and 76.0% (38/50) of samples were negative. The molecular consensus was not seen in 4.0% (02/50) of samples. Conclusions These results suggest that a possible interaction between human and simian malaria coming from a zoonotic cycle cannot be discarded because simians that live in the areas of the Atlantic Forest could play a role as a reservoir for Plasmodium. (AU)

• Processo FAPESP: 08/51278-0 - Detecção dos agentes etiológicos da malária autóctone em símios capturados na Mata Atlântica do Estado de São Paulo
• Beneficiário: Tasciane Yamasaki
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 05/56055-1 - Deteccao dso agentes etiologicos da malaria autoctone em simios capturados na mata atlantica dos estados do espirito santo e sao paulo
• Beneficiário: Rosely dos Santos Malafronte
• Modalidade de apoio: Auxílio à Pesquisa - Regular