Therapeutic DNA Vaccine Reduces Schistosoma mansoni-Induced Tissue Damage through Cytokine Balance and Decreased Migration of Myofibroblasts

Autor(es)

Frantz, Fabiani Gai ^[1] ; Ito, Toshihiro ^[2] ; Cavassani, Karen Angelica ^[2] ; Hogaboam, Cory M. ^[2] ; Silva, Celio Lopes ^[3] ; Kunkel, Steven L. ^[2] ; Faccioli, Lucia H. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Clin Anal Toxicol & Bromatol, Ribeirao Preto Coll Pharmaceut Sci, BR-14049 Ribeirao Preto, SP - Brazil

^[2] Univ Michigan, Sch Med, Dept Pathol, Ann Arbor, MI - USA

^[3] Univ Sao Paulo, Dept Biochem & Immunol, Ribeirao Preto Coll Med, BR-14049 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: American Journal of Pathology; v. 179, n. 1, p. 223-229, JUL 2011.
• Citações Web of Science: 6

Resumo

Helminths are known to elicit a wide range of immunomodulation characterized by dominant Th2-type immune responses. Our group previously showed that a DNA vaccine encoding the mycobacterial 65-kDa heat shock protein (DNA-hsp65) showed immunomodulatory properties. We also showed, using a helminth-tuberculosis (TB) co-infection model, that the DNA-hsp65 vaccine protected mice against TB. We next investigated the mechanistic role of the vaccine during helminth-TB co-infection. Clinically, helminth infection causes type 2 granulomas in the lung. Mice were immunized with DNA-hsp65 while they were submitted to the type 2 granuloma induction protocol by Schistosoma mansoni eggs infusion. In this work we investigated the effects of DNA-hsp65 on the pathology and inunune response during the development of type 2 granuloma induced by S.mansoni eggs. Histologic analyses of lung parenchyma showed that the DNA-hsp65 vaccine protected mice against exacerbated fibrosis induced by Schistosoma eggs, and decreased the size of the granulomas. These changes were correlated with a reduction in the number of T cells specific for the egg antigens in the lung and also with modulation of Th2 cytokine expression. Taken together, our results showed that the adjuvant properties of the DNA-hsp65 vaccine regulated the immune response in this Th2 model, and resulted in a preserved lung parenchyma. (Am J Patbol 2011, 179:223-229; DOI: 10.1016/j.ajpath.2011.03.012) (AU)

• Processo FAPESP: 03/12887-8 - Estudo do efeito terapêutico da vacina DNA-hsp65 na co-infecção por Toxocara canis e Mycobacterium tuberculosis
• Beneficiário: Fabiani Gai Frantz
• Linha de fomento: Bolsas no Brasil - Doutorado