Levels of copper in Nile tilapia from Brazil

Autor(es)

Neves, Renato C. F. ^[1] ; Moraes, Paula M. ^[2] ; Ferrari, Jeisson E. M. ^[1] ; Lima, Paula M. ^[1] ; Santos, Felipe A. ^[2] ; de Castro, Gustavo Rocha ^[2] ; Barros, Margarida M. ^[1] ; Padilha, Pedro M. ^{[2, 3]}

Afiliação do(s) autor(es)

^[1] UNESP, Fac Med Vet & Zootecnia, Programa Posgrad Zootecnia, BR-15385000 Botucatu, SP - Brazil

^[2] UNESP, Inst Biociencias, Dept Quim & Bioquim, BR-18618000 Botucatu, SP - Brazil

^[3] Univ Estadual Campinas, Inst Nacl Ciencia & Tecnol Bioanalit, BR-13084971 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FOOD ADDITIVES & CONTAMINANTS PART B-SURVEILLANCE; v. 4, n. 4, p. 238-243, 2011.
• Citações Web of Science: 3

Resumo

The purpose of this work was to determine the concentration of copper in samples of farmed Nile tilapia (Oreochromis niloticus L.) fillets purchased in the city of Botucatu (Sao Paulo, Brazil) and in fillet and liver samples of Tilapia fed diets supplemented with different concentrations of Cu from the Laboratory of Aquatic Organism Nutrition/FMVZ-UNESP (Botucatu, Brazil). The fillet samples were prepared by lyophilisation and cryogenic grinding into particles smaller than 60 mm, and copper was extracted ultrasonically using 0.10 mol l(-1) HCl as extraction solution. Copper determination was performed by graphite furnace atomic absorption spectrometry (GFAAS) with optimised temperatures of drying, pyrolysis, atomisation and cleaning. Palladium nitrate was injected into the samples as a chemical modifier and tungsten as a permanent modifier. Copper concentrations of 0.70-1.60mg kg(-1) were found, which are in line with Brazilian regulations. The accuracy and precision of the copper concentrations determined in this study were evaluated using certified standard Lake Michigan fish tissue (NIST SRM 1947) (AU)

• Processo FAPESP: 03/13362-6 - Determinação de Ca, Cu, Cr, Fe, Mn, Se e Zn em suspensões de rações e fezes de peixes por espectrometria de absorção atômica em forno de grafite para estimativa da biodisponibilidade relativa de nutrientes minerais utilizados em piscicultura
• Beneficiário: Pedro de Magalhães Padilha
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/59778-0 - Desenvolvimento de metodologias analíticas para avaliação de metaloproteínas de tilápia do Nilo (Oreochromis niloticus)
• Beneficiário: Pedro de Magalhães Padilha
• Linha de fomento: Auxílio à Pesquisa - Regular