Proteomic Characterization of the Human FTSJ3 Preribosomal Complexes

Autor(es)

Simabuco, Fernando M. ^[1] ; Morello, Luis G. ^[1] ; Barbosa Aragao, Annelize Zambon ^[1] ; Paes Leme, Adriana Franco ^[1] ; Zanchin, Nilson I. T. ^[2]

Afiliação do(s) autor(es)

^[1] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Biociencias, BR-13083970 Campinas, SP - Brazil

^[2] Fundacao Inst Oswaldo Cruz FIOCRUZ, Inst Carlos Chagas, BR-81350010 Curitiba, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROTEOME RESEARCH; v. 11, n. 6, p. 3112-3126, JUN 2012.
• Citações Web of Science: 6

Resumo

In eukaryotes, ribosome biogenesis involves excision of transcribed spacer sequences from the preribosomal RNA, base and ribose covalent modification at specific sites, assembly of ribosomal proteins, and transport of subunits from the nucleolus to the cytoplasm where mature ribosomes engage in mRNA translation. The biochemical reactions throughout ribosome synthesis are mediated by factors that associate transiently to the preribosomal complexes. In this work, we describe the complexes containing the human protein FTSJ3. This protein functions in association with NIP7 in ribosome synthesis and contains a putative RNA-methyltransferase domain (FtsJ) in the N-terminal region and two uncharacterized domains in the central (DUF3381) and C-terminal (Spb1\_C) regions. FLAG-tagged FTSJ3 coimmunoprecipitates both RPS and RPL proteins, ribosome synthesis factors, and proteins whose function in ribosome synthesis has not been demonstrated yet. A similar set of proteins coimmunoprecipitates with the Spb1\_C domain, suggesting that FTSJ3 interaction with the preribosome complexes is mediated by the Spb1\_C domain. Approximately 50% of the components of FTSJ3 complexes are shared by complexes described for RPS19, Par14, nucleolin, and NOP56. A significant number of factors are also found in complexes described for nucleophosmin, SBDS, ISG20L2, and NIP7. These findings provide information on the dynamics of preribosome complexes in human cells. (AU)

• Processo FAPESP: 06/02083-7 - Caracterizacao funcional de proteinas envolvidas no controle da expressao genica.
• Beneficiário: Nilson Ivo Tonin Zanchin
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 03/06299-6 - Estudos da relação estrutura e função da proteína Nip7p e análise do seu papel no metabolismo de RNA em Saccharomyces cerevisiae e Homo sapiens
• Beneficiário: Patricia Pereira Coltri
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/58371-3 - Determinação do papel das proteínas Ftsj3 e CXorf34 na síntese de ribossomos em células humanas
• Beneficiário: Luis Gustavo Morello
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/57110-4 - Caracterização da função da proteína ISG95 no metabolismo de RNA e de seu papel na resposta à infecção viral
• Beneficiário: Fernando Moreira Simabuco
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado